权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Application of Optical Biopsy by Fluorescent Endoscopic Observation using a New Photosensitizer

新型光敏剂荧光内窥镜观察光学活检的应用

基本信息

批准号：
11672293
负责人：
MIYOSHI Norio
金额：
$ 0.45万
依托单位：
Fukui Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

We investigated the photodynamic macrotic diagnosis (PDMD) of a cancer by a new semiconductor laser (iLS-LD400 model, 405 nm, 5 mW) using the photosensitizer protoporphyrin-IX (Pp-IX) generated in vivo from 5-aminolevulinic acid=5-ALA which has an affinity for the squamous cell carcinoma (SCC) tumor transplanted on the back of C3H/He mouse. In the result, it was found the specific affinity of the Pp-IX in the SCC tumor from the spectroscopic data that the Pp-IX fluorescent intensity in the tissue was enhanced 2 times by the ultrasound irradiation (47 kHz, 50 W, 10 min).Furthermore, it was also investigated the fluorescent spectroscopic analysis of the Pp-IX incorporated in the clinical glyoblastoma and astrocytoma tissue sample which were frozen and were cryo-sectioned at 10 μm. It was found that the Soret vand peak was presented at 405 nm from the excitation spectra. The new semiconductor laser light (iLS-LD400 model, IHI., 405 nm, 5 mW) was same as that of the Soret vand peak. It is very convenient to do a macrotic diagnosis to detect the Pp-IX incorporated into the tumor. Furthermore, it was also found that the best suited wavelengths were 572-606, 630 and 670 nm to treat the tumor photodynamically after the cancer operation from the excitation spectra of these samples.In conclusion, it will be established to do the endoscopic diagnosis for the tumor incorporated 5-ALA in the Clinical brain tumor tissue using a new semiconductor laser (iLS-LD400 model, IHI) and to treat the brain and/or the other epithelium tumors by the lamp at more longer wavelength from 570 nm.

研究了一种新型半导体激光器(ILS-LD400型，波长405 nm，功率5 mW)对肿瘤的光动力学宏观诊断(PDMD)。结果发现，PP-IX在鳞状细胞癌组织中的特异性亲和力在超声(47 kHz，50W，10min)作用下增强了2倍。此外，还研究了在10μm处冷冻和冷冻的临床成纤维细胞瘤和星形细胞瘤组织标本中PP-IX的荧光光谱分析，从激发光谱中发现Sorevand峰出现在405 nm处。新的半导体激光(ILS-LD400型，IHI，405 nm，5 mW)与Soret vand峰的激光相同。检测PP-IX在肿瘤中的整合情况，对于进行宏观诊断是非常方便的。此外，从这些样品的激发光谱中还发现了572-606、630和670 nm是肿瘤术后光动力学治疗的最佳波长。综上所述，这将为利用新型半导体激光器(ILS-LD400型号，IHI)对临床脑肿瘤组织中掺入5-ALA的肿瘤进行内窥镜诊断和利用波长更长的570 nm的灯治疗脑部和/或其他上皮性肿瘤奠定基础。