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A new method of measurement of the UV protection efficiency of sunscreen goods in the biological aspect

生物方面测定防晒品紫外线防护效率的新方法

基本信息

批准号：
11680103
负责人：
YAMANO Haruko
金额：
$ 1.92万
依托单位：
Ochanomizu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680103/
关键词：
Ultraviolet rays (UV)Ozone layer Flon Carbon dioxide p53 protein Apoptosis UV protection goods SPF value 地球環境皮膚傷害皮膚癌培養細胞株皮膚障害癌抑制遺伝子紫外線遮蔽効果

项目摘要

In recent years, ozone layer that have sheltered living bodies on the earth from harmful effects of ultraviolet rays (UV) is broken by flon and carbon dioxide etc.Therefore sunscreen goods with the properties of protection of human skins, against UV have been developted one after another. The determination of the cutting ratio of UV protection of these manufactures have been measured by photometry or sun protection factor (SPF) value, which is the only biological measurement. But the measurement by optical instrument not reflect biological responses, and measurement of SPF value is dangerous because this is carried out with skin of healthy subjects.Accordingly, in the determination of the cutting ratio of UV protection goods, we have planned the new method without subjects. After UV irradiation, we assayed the decrease of the activity of the enzyme solution placed in the culture dishes covered with or without UV protection goods.This corresponded to the results of photometry or measurement of SPF value.Recently, it was proved that tumor-suppressor protein p53 acts as the key protein in the regulation of cell cycle or apoptosis, a mechanism for eliminating unwanted cells from the cell community in multicellular organisms. The other, recent studies indicate that levels of p53 increase rapidly in response to DNA damage induced by UV irradiation.Then, we have planned to measure levels of p53 protein and apoptosis with various established human cell lines. These cells were UV irradiated in culture dishes covered with or without UV cutting goods. The detection of the apoptosis of these cultured cells were measured by in situ apoptosis detection kits.It was suggested that this newmethod could substitute for photometry or SPF method.

近年来，保护地球上的生物免受紫外线（UV）有害影响的臭氧层被臭氧和二氧化碳等破坏，因此具有保护人类皮肤抵抗UV的性质的防晒产品已经相继开发。这些制造商的紫外线防护切割率的确定是通过光度法或防晒系数（SPF）值来测量的，这是唯一的生物测量方法。但是，用光学仪器测定不能反映生物学反应，而且SPF值的测定是用健康人的皮肤进行的，因此有危险性。因此，在紫外线防护品的分割率测定中，我们计划了无受试者的新方法。紫外线照射后，将酶液置于覆盖或未覆盖紫外线防护物品的培养皿中，检测酶液活性的降低，这与光度法或SPF值测定的结果相一致。最近，抑癌蛋白p53被证实是调节细胞周期或凋亡的关键蛋白，从多细胞生物体的细胞群落中清除不需要的细胞的机制。另一方面，最近的研究表明，p53的水平迅速增加，响应于DNA损伤的UV照射。然后，我们计划用各种已建立的人类细胞系来测量p53蛋白水平和凋亡。这些细胞在覆盖有或没有UV切割物品的培养皿中进行UV照射。用细胞凋亡原位检测试剂盒检测培养细胞的凋亡情况，提示该方法可替代光度法或SPF法。