Ultrastructural study on the similarity and diversity of allergen pathway in the digestive tract of suckling rats

乳鼠消化道过敏原途径相似性和多样性的超微结构研究

• 批准号: 11680147
• 负责人: MATSUGUMA Miki
• 金额: $ 0.9万
• 依托单位: NAKAMURA GAKUEN UNIVERSITY, FACULTY OF HOME ECONOMICS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680147/
• 关键词: food allergen; ultrastructure; intestine; suckling rat; 吸収上皮細胞

Con A-HRP, PNA-HRP, LCA-HRP and RCA_<120>-HRP bound strongly to components of the glycocalyx on the plasma membrane of the microvilli, apical coated pits and the small tubular pits at the base of the microvilli of the absorptive cells. The lectin-conjugates on the plasma membranes were endocytosed from the coated pits and small tubular pits, and then transported into coated vesicles, vesicles and small tubules. Lectin-HRP conjugates were later found within the intercellular space. In contrast, SBA-HRP and DBA-HRP bound weakly to the plasma membranes. The absorptive cells demonstrated little uptake or transepithelial transport.When WGA-HRP was injected into the intestinal lumen in vivo, the jejunal absorptive cells formed a deep and wide apical invagination at the base of the microvilli. WGA-HRP bound strongly to the glycocalyx on the plasma membranes however was almost entirely transported into the early endosomes, late endosomes, multivesicular bodies and lysosomes.These studies suggest the similarity and diversity of invasion of allergen in the small intestinal absorptive cells of the suckling rats.

ConA-HRP、PNA-HRP、LCA-HRP和RCA_<120>-HRP与吸收细胞微绒毛质膜上的糖萼成分、顶端被膜小凹和基部小管小凹有较强的结合。质膜上的凝集素结合物从被膜小凹和小管小凹中被内吞，然后转运到被膜小泡、囊泡和小管中。后来在细胞间隙内发现凝集素-HRP结合物。相反，SBA-HRP和DBA-HRP与质膜的结合较弱。在体肠腔注射WGA-HRP时，空肠吸收细胞在微绒毛基部形成一个深而宽的顶端内陷。WGA-HRP与质膜上的糖萼结合较强，但几乎全部转运到早期内体、晚期内体、多泡体和溶酶体中，提示过敏原在乳鼠小肠吸收细胞中侵袭的相似性和多样性。

项目成果

M.Fyjita,R.Baba,R.Tanaka,S.Nozaki,M.Miyoshi: "Similarity and Diversity of an Apical Endocytic Membrane System in the Absorptive Cells of the Rat Small and Large Intestine"Digsetion&Absorption. 22(2). 9-14 (1999)

M.Fyjita，R.Baba，R.Tanaka，S.Nozaki，M.Miyoshi：“大鼠小肠和大肠吸收细胞顶端内吞膜系统的相似性和多样性”消化

R.Baba,M.Fujita,R.Tanaka,M.Miyoshi.: "Cellular differentiation of absorptive cells in the neonatal colon : an electron microscopic study"MEDICAL ELECTRON MICROSCOPY. 32・2. 105-113 (1999)

R.Baba、M.Fujita、R.Tanaka、M.Miyoshi.：“新生儿结肠中吸收细胞的细胞分化：电子显微镜研究”医学电子显微镜 32・2（1999）。

M.Fujita, R.Baba, R.Tanaka, S.Nozaki, M.Miyoshi: "Similarity and Diversity of an Apical Endocytic Membrane System in the Absorptive Cells of the Rat Small and Large Intestine"Digestion & Absorption. 22. 9-14 (2000)

M.Fujita、R.Baba、R.Tanaka、S.Nozaki、M.Miyoshi：“大鼠小肠和大肠吸收细胞顶端内吞膜系统的相似性和多样性”消化

M.Fujita,R.Baba,R.Tanaka,S.Nozaki,M.Miyoshi: "Similarity and Diversity of an Apical Endocytic Membrane System in the Absorptive Cells of the Rat Small and Large Intestine"Digestion & Absorption. 22・2. 9-14 (2000)

M.Fujita、R.Baba、R.Tanaka、S.Nozaki、M.Miyoshi：“大鼠小肠和大肠吸收细胞顶端内吞膜系统的相似性和多样性”消化与吸收 22・2。 9-14 (2000)

M.Fujita,R.Baba,S.Nozaki,M.Miyoshi.: "Basolateral Endocytic pathways in the Epithelial Cells of Rat Stomach in vivo"Digestion & Absorption. 23・2(in press). (2001)

M.Fujita、R.Baba、S.Nozaki、M.Miyoshi.：“体内大鼠胃上皮细胞的基底外侧内吞途径”23・2（印刷中）。