Study on the production of medicinal plants characteristic of Vietnam based on the field work.

基于田野调查的越南特色药用植物生产研究

• 批准号: 11793018
• 负责人: HONDA Gisho
• 金额: $ 4.1万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11793018/
• 关键词: Vietnam; cinnamon; agarwood; Cinnamomum; Aquilaria; field work; incense; medicinal plants

We conducted the field research on medicinal plants in Vietnam 3 times . The first trip was in the southern part, the second in the northern part, and the third in the southern and middle part. We focused on cinnamon ( Cinnamomum sp. ), agarwood (Aguilaria sp. ) and the traditional medicines used in Vietnam, and collected samples for analyses as well as information about them. Various experiments were performed on all these samples to reveal the following results. Cinnamon : Vietnamese cinnamon consists of 2 types, the YB type produced in northern part and the MN type in southern part, and their characteristics are much different, which, according to the results of the phylogenetic analyses and the information gathered in Vietnam, is due to the difference of original plant species. Agarwood : Aguilaria, the original tree of agarwood, is cultivated in the middle part of Vietnam, and there we heard about a method for artificial producing agarwood. On the other hand, seedlings of Aguilaria have been grown in the greenhouse built in Graduate School of Pharmaceutical Sciences, Kyoto University. Comparison of nucleic acid sequences for phylogenetic analysis and induction of cell culture for experiments under a germ free condition were performed using fresh leaves of them. Branches of Aguilaria trees in the greenhouse were experimentally injured in order to induce production of resin, and a little amount of resin containing some fragrant compounds was recognized. Trunk of Taiwanese Aquilaria sinensis which had been injured by the typhoon few years ago, was phytochemically analyzed to isolate several species of chromones. Traditional Medicines in Vietnam : Traditional medicines used in Vietnam were extracted to measure trypanocidal activity, and Alpinia galanga and Sophora suffruticosa, which showed strong activity, were submitted to further experiments to isolate active compounds.

我们对越南药用植物进行了3次实地考察。第一次是南部，第二次是北部，第三次是南部和中部。我们重点研究了肉桂（Cinnamomum sp.）、沉香（Aguilaria sp.）和越南使用的传统药物，并收集了样品进行分析以及有关它们的信息。对所有这些样品进行了各种实验以揭示以下结果。肉桂：越南肉桂分为2个类型，北部产YB型，南部产MN型，其特征差异很大，根据系统发育分析结果和越南收集的信息，这是由于原始植物种类的差异所致。沉香：沉香的原始树种沉香，种植在越南中部，在那里我们听说了人工生产沉香的方法。另一方面，京都大学药学研究生院建造的温室中培育了沉香的幼苗。使用它们的新鲜叶子进行用于系统发育分析的核酸序列的比较以及在无菌条件下实验的细胞培养物的诱导。为了诱导树脂的产生，对温室中的沉香树的树枝进行了实验损伤，并发现了少量含有某些芳香化合物的树脂。对几年前被台风损坏的台湾沉香树干进行植物化学分析，分离出几种色酮。越南的传统药物：提取越南使用的传统药物来测量杀锥虫活性，并将表现出强活性的高良姜和槐花进行进一步的实验以分离活性化合物。