Health benefits and genetic analysis of millets, and food developments in cooperation with community food companies

小米的健康益处和遗传分析，以及与社区食品公司合作的食品开发

• 批准号: 11794012
• 负责人: NISHIZAWA Naoyuki
• 金额: $ 7.3万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794012/
• 关键词: millet; health benefit; commercialization; food development; variety discrimination; millet bread; sorghum pastry; 肝障害抑制; 生理機能性; 雑穀和菓子; 雑穀農機具; 雑殻; プロラミン; 雑殻遺伝子; AFLP; アミノ酸配列

(1). The diets containing Japanese and proso millets protein and the whole grain lowered plasma total and LDL-cholesterol levels and increased output of bile acids into feces in rats. Further the diet of black Japanese millet protein suppressed serum levels of OVA-specific IgE.(2). Foxtail millets from lwate were discriminated from those from Hokkaido, Nagoya, China and Korea by AFLP procedure.(3). A new disease causing leaf blight on proso millet (Panicum miliaceum L. ) was found on all plants in a field of Karumai, Iwate in August, 2000. A species of Fusarium was isolated from many infected leaves. This fungus was found to be pathogenic on P. miliaceum by inoculation of plant and identified as F. roseum This is the first report of Fusarium disease on P. miliaceum in Japan.(4). In order to clear whether the variants of seed prolamin in foxtail millet are new type, they were crossed with the strains having standard prolamin bands. Prolamin of seeds obtained by the hybridization are under investigation, but the hybrids are not obtained until now. When amino acid composition of seed proteins in soybean which accumulated several mutant genes lacking subunit of globulin was analyzed, they showed increase of arginine content about two time.(5). A procedure to remove effectively weeds in Japanese millet field by machine was found.(6). A new millet bread and a Japanese pastry were developed and commercialized in cooperation with community food companies.

（一）.含有日本和Proso小米蛋白质和全谷物的饮食降低了血浆总胆固醇和LDL-胆固醇水平，并增加了大鼠粪便中胆汁酸的输出。此外，黑日本小米蛋白的饮食抑制血清中的OVA特异性IgE水平。（二）、利用AFLP技术对岩手县的谷子与来自北海道、名古屋、中国和韩国的谷子进行了鉴别。（三）、谷子（Panicum miliaceum L.）2000年8月，在岩手县Karumai的一片田野中，在所有植物上都发现了这种细菌。从许多感染的叶片中分离到一种镰刀菌。通过植物接种试验，发现该真菌对谷子疫霉具有致病性，经鉴定为F。这是首次在日本报道粟粒拟青霉镰刀菌病。（四）、为了明确谷子种子醇溶蛋白变异体是否为新类型，将其与具有标准醇溶蛋白带的品系杂交。杂交获得的种子醇溶蛋白的研究正在进行中，但到目前为止还没有获得杂交种。对积累了几个球蛋白亚基缺失突变基因的大豆种子蛋白氨基酸组成分析表明，精氨酸含量增加了约2倍。（五）、探索出一种能有效地机械清除日本谷子田杂草的方法。（六）、与社区食品公司合作开发了一种新的小米面包和一种日式糕点，并将其商业化。

项目成果

Lei Zhang, Yoshihito Takahata, Yo-ichi Takahashi, Takashi Nagasawa, Yoshiaki Ito, Naoyuki Nishizawa, Myeong-Rak Choi, You-Young Choi, and Yi Min Wei: "Proteins of foxtail millets and DNA polymorphisn by AFLP procedure"Annual Meeting of Japan Society of Bi

Lei Zhang、Yoshihito Takahata、Yo-ichi Takahashi、Takashi Nagasawa、Yoshiaki Ito、Naoyuki Nishizawa、Myeong-Rak Choi、You-Young Choi 和 Yi Min Wei：“通过 AFLP 程序进行小米的蛋白质和 DNA 多态性分析”年会

Tomita Y.: "Kinetics of Interactions of Sendai Virus Envelope Glycoproteins, F and HN, with Endoplasmic Reticulum=Resident Molecular Chaperones, BiP, Calnexin, and Calreticulin"J. Biochem.. 126. 1090-1100 (1999)

Tomita Y.：“仙台病毒包膜糖蛋白、F 和 HN 与内质网 = 驻留分子伴侣、BiP、钙联蛋白和钙网蛋白相互作用的动力学”J。

西澤直行(分担執筆): "地域農産物の品質・機能性成分総覧"(株)サイエンスフォーラム. 426-430 (2000)

Naoyuki Nishizawa（合着）：《地方农产品的品质和功能成分概述》科学论坛有限公司 426-430（2000）

K. Oikawa, T. Nagasawa and N. Nishizawa: "Effects of dietary millet protein on lipid metabolism in hamsters"Annual Meeting of Japan Society of Bioscience, Biotechnology and Agrochemistry. Abstract. (2000)

K. Oikawa、T. Nagasawa 和 N. Nishizawa：“膳食小米蛋白对仓鼠脂质代谢的影响”日本生物科学、生物技术和农业化学学会年会。

Lei Zhang, Kiyomi Oikawa, Takashi Nagasawa, Yoshiaki Ito, Naoyuki Nishizawa: "Effects of dietary sorghum on lipid metabolism and antioxidative activity in rats"Annual Meeting of Japanese Society of Nutrition and Food Science. Abstract. (2000)

张雷、及川清美、长泽隆、伊藤义明、西泽直之：“膳食高粱对大鼠脂质代谢和抗氧化活性的影响”日本营养食品学会年会。