Basic study for modulation of macrophage function by ELF electric field.

ELF电场调节巨噬细胞功能的基础研究。

• 批准号: 11834001
• 负责人: SHIMIZU Koichi
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11834001/
• 关键词: ELF electric field; immune response; immunomodulation; macrophage; phagocytosis; displacement current; flow cytometry; 貧食能; 電気刺激_

To pursue the possibility of immunomodulation by ELF electric field exposure, the effects of the electro-stimulation on phagocytosis of macrophages were investigated in in vitro experiments. The magnitude of the electric field was in the same level as the commercial apparatus for the electric field therapy. Through this study, the following results were obtained.1. Direct stimulation with electric currentThe phagocytosis of peritoneal exudate macrophages from a mouse was studied. As phagocytized particles, fluorescent latex beads were used. The current density and the duration of the stimulation were 0.1 or 1A/m^2, and from 10 sec to 1 hour, respectively. In microscope observation, apparent reduction of phagocytosis was observed in stimulated macrophages. There was not significant difference among the results in different exposure conditions examined.2. Development of exposure system for indirect stimulationIn the commercial apparatus for the electric field therapy, the displacement cu … More rrent is generated in the human body. To simulate this indirect stimulation, the experimental container was designed to stimulate macrophages with displacement current. To supply the voltage to the container, the power source of the commercial apparatus for the electric field therapy was used. With this source, the current density of 0.35 A/m^2 was applied to the container.3. Stimulation with the displacement currentThe effect of displacement current on the phagocytosis of the macrophages was studied. The current density and the duration of stimulation were 0.35 A/m^2 (50Hz) and 30min or 1 h, respectively. An assay was made on the activity of phagocytosis in the flow cytometry. The suppression of phagocytosis was observed in stimulated macrophages. The suppression was apparent particularly in the macrophages with active phagocytosis.The results of this study suggested the possibility to suppress the phagocytosis of macrophages. This suppression can be made with the commercial apparatus for the electric field therapy. The suppression of macrophage activity will result in the modification of the total immunological response through the process of antigen presentation. Therefore, the feasibility of immunomodulation by the ELF electro-stimulation has been verified through this study. Less

为了探讨极低频电场对巨噬细胞免疫功能的调节作用，本研究在体外实验中观察了极低频电场对巨噬细胞吞噬功能的影响。电场强度与市售电场治疗仪相同。通过本研究，获得了以下结果.用电流直接刺激小鼠腹腔渗出液巨噬细胞，观察其吞噬功能。作为吞噬的颗粒，使用荧光乳胶珠。电流密度和刺激时间分别为0.1或1A/m^2，从10秒到1小时。在显微镜下观察，在刺激的巨噬细胞中观察到明显的吞噬功能降低。不同暴露条件下的检测结果无显著性差异.间接电刺激曝光系统的研制 ...更多信息 在人体内产生电流。为了模拟这种间接刺激，实验容器被设计成用置换电流刺激巨噬细胞。为了向容器提供电压，使用用于电场治疗的商业设备的电源。利用该源，向容器施加0.35 A/m^2的电流密度。位移电流刺激研究了位移电流对巨噬细胞吞噬功能的影响。电流密度为0.35A/m^2（50 Hz），刺激时间为30 min或1h。用流式细胞仪测定吞噬活性。在刺激的巨噬细胞中观察到吞噬抑制。这种抑制作用在吞噬活性较强的巨噬细胞中尤为明显，提示了抑制巨噬细胞吞噬功能的可能性。这种抑制可以用用于电场治疗的商业设备来进行。巨噬细胞活性的抑制将导致通过抗原呈递过程的总免疫应答的改变。因此，本研究证实了极低频电刺激免疫调节的可行性。少

项目成果

清水久恵 他: "ELF電界曝露に伴う生理的変化に関する基礎的検討-ヒト体表における血流変化の可能性-"電子情報通信学会技術研究報告. EMCJ99-97. 49-53 (1999)

Hisae Shimizu 等人：“与 ELF 电场暴露相关的生理变化的基础研究 - 人体表面血流变化的可能性 -”IEICE 技术研究报告 49-53（1999）。

H.Shimizu, et al.: "Effects of ELF electric field on physiological function"Proceedings of ISAP 2000. in press. (2000)

H.Shimizu 等人：“ELF 电场对生理功能的影响”，ISAP 2000 年报，出版中。

T.Shimooka, et al.: "ELF electrostimulation on macrophages : possibilty of immunomodulation by electric field"Proceedings of the 22nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. (CR-ROM). (2000)

T.Shimooka 等人：“巨噬细胞上的 ELF 电刺激：通过电场进行免疫调节的可能性”IEEE 医学和生物学工程学会第 22 届国际年会论文集。

H.Shimizu, J.Arisawa and K.Shimizu: "Fundamental study on physiological change associated with ELF electric field exposure=possibility of blood flow change in human body="Technical Report of IEICE. EMCJ99-97. 49-53 (1999)

H.Shimizu、J.Arisawa 和 K.Shimizu：“与 ELF 电场暴露相关的生理变化的基础研究=人体血流变化的可能性=”IEICE 技术报告。

H.Shimizu and K.Shimizu: "Experimental analysis of the human perception threshold of a DC electric field"Med.Biol.Eng.Comp.. 37. 727-732 (1999)

H.Shimizu 和 K.Shimizu：“直流电场的人类感知阈值的实验分析”Med.Biol.Eng.Comp.. 37. 727-732 (1999)