权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The role of cardiovascular aldosterone for the vascular hypertrophy and atherosclerosis

心血管醛固酮在血管肥大和动脉粥样硬化中的作用

基本信息

批准号：
11838004
负责人：
MIYAMORI Isamu
金额：
$ 2.05万
依托单位：
Fukui Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Aldosterone and other mineralocorticoids are synthesized in the cardiovascular tissues. In the genetically hypertensive rat, aldosterone synthase genes (CYP11 B2) are expressed in the heart and up-regulated by angiotensin II (Ang II). Since aldosterone potentiates the hypertrophic effect of Ang II through mineralocorticoid receptors (MRs) which are present in the heart and vessels, it is suggested that the systemic and local renin-angiotensin-aldosterone system contribute to the cardiac hypertrophy in an accelerated fashion. Using PCR-RT we analyzed all enzymes involved in corticosteroids in human vascular cells cultivated from pulmonary arteries. Genes encoding these enzymes were detected with the exception of cholesterol side chain cleavage and 11β-hydroxylase genes. Thus aldosterone may be synthesized from the circulating precursor steroids in vascular cells, which is different from the steroidogensis in the adrenal cortex. CYP11B2 expression in both endothelial cells and smooth muscle cells is stimulated by 10-9 M Ang II, supporting the role of aldosterone for Ang II induced vascular remodeling. In spite of these evidence in arteries, the existence and functional role of local aldosterone is controversial in the human heart. Positive immunohistochemical staining of MRs in the failling human adult heart favors the clear role of aldosterone in such circumstances regardless of whether it is synthesized in the adrenal or in the heart.

醛固酮和其他盐皮质激素在心血管组织中合成。在遗传性高血压大鼠中，醛固酮合成酶基因（CYP 11 B2）在心脏中表达并被血管紧张素II（Ang II）上调。由于醛固酮通过存在于心脏和血管中的盐皮质激素受体（MR）增强Ang II的肥大作用，因此认为全身和局部的肾素-血管紧张素-醛固酮系统以加速的方式促进心脏肥大。使用PCR-RT，我们分析了所有的酶参与皮质类固醇在人血管细胞培养肺动脉。除胆固醇侧链裂解和11β-羟化酶基因外，检测到编码这些酶的基因。因此，醛固酮可能是在血管细胞中由循环的前体类固醇合成的，这与肾上腺皮质中的类固醇合成不同。内皮细胞和平滑肌细胞中的CYP 11 B2表达被10-9 M Ang II刺激，支持醛固酮在Ang II诱导的血管重塑中的作用。尽管在动脉中有这些证据，但局部醛固酮在人类心脏中的存在和功能作用仍存在争议。在衰竭的成人心脏中，MR的阳性免疫组织化学染色有利于醛固酮在这种情况下的明确作用，无论它是在肾上腺还是在心脏中合成。