Analysis of molecular mechanism and cell-cell interactions in the establishment of left-right asymmetry

左右不对称建立的分子机制和细胞间相互作用分析

• 批准号: 11680714
• 负责人: TAMURA Koji
• 金额: $ 2.37万
• 依托单位: TOHOKU University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680714/
• 关键词: Left-right asymmetry; retinoic acid; Pitx2; morphogenesis; flounder; 左右軸

Left-right asymmetry in heart and intestinal organs of vertebrates is produced in the process of their development. We investigated that shh, Pitx2 and retinoic acid are involved in this process, and that they are responsible for the left handedness. In particular, we cloned Pitx2 gene from zebrafish and analyzed its expression pattern, function, gene regulations, and interaction with retinoic acid (Tsukui et al., 1999).On the other hand, we cloned several members of T-box gene family, which are largely involved in organ for mation and patterning, in order to examine their functions on left-right asymmetry. We analyzed their expression patterns in zebrafish embryo (Yonei-Tamura et al., 1999 ; Tamura et al., 1999), and confirmed an important role of Tbx5/4 in chick embryo (Rodriguez-Esteban et al., 1999).We also started a project on flounder and left-right asymmetry. We examined and evaluated the visual chiasma as a significant phenotype of left-right asymmetry, and used as a guidepost of left-right asymmetry. We also cloned several PCR fragments of key genes on left-right asymmetry, includeing Pitx2, fgf8, Noda11, and msx (2) from the flounder.The above results were published in a review article (Tamura et al., 1999).

脊椎动物的左右不对称性是在其发育过程中产生的。我们研究了SHH，PITX2和视黄酸参与此过程，并且它们负责左手。特别是，我们从斑马鱼中克隆了PITX2基因，并分析了其表达模式，功能，基因调节以及与视黄酸的相互作用（Tsukui等，1999）。另一方面，我们克隆了T-box基因家族的几个成员，这些成员主要参与了机构和模式，以便在其左右效果，以便在左右效果。我们分析了它们在斑马鱼胚胎中的表达模式（Yonei-Tamura等，1999; Tamura等，1999），并证实了TBX5/4在鸡肉胚胎中的重要作用（Rodriguez-Esteban等，1999）。我们还启动了Flounder and flowounder和左右的项目。我们检查并评估了视觉智能作为左右不对称的重要表型，并用作左右不对称的指南。我们还克隆了左右不对称的关键基因的几个PCR片段，包括来自比目鱼的pitx2，FGF8，NODA11和MSX（2）。以上结果发表在评论文章中（Tamura等，1999）。

项目成果

Tsukui,T.,Capdevila,J.,Tamura K.et al.: "Multiple left-right asymmetry defects in Shh-/-mutant mice unveil a convergence of the Shh and Retinoic Acid pathways in the control of Lefty-1"Proc. Natl. Acad. Sci.. 96. 11376-11381 (1999)

Tsukui,T.、Capdevila,J.、Tamura K.等人：“Shh-/-突变小鼠中的多个左右不对称缺陷揭示了控制 Lefty-1 的 Shh 和视黄酸途径的收敛”Proc

Yonei-Tamura,S.,Tamura,K.,Tsukui,T.,and Belmonte,J.C.: "Spatially and temporal-restricted expression of two T-box genes during zebrafish embryogenesis."Mechanism of Development. 80. 219-221 (1999)

Yonei-Tamura,S.、Tamura,K.、Tsukui,T. 和 Belmonte,J.C.：“斑马鱼胚胎发生过程中两个 T 盒基因的空间和时间限制性表达。”发育机制。

Tamura, K., Yonei-Tamura, S., and Izpisua Belmonte, J.C.: "Molecular basis of left-right asymmetry."Development, Growth & Differentiation. 61. 645-656 (1999)

Tamura, K.、Yonei-Tamura, S. 和 Izpisua Belmonte, J.C.：“左右不对称的分子基础。”发育、生长

Tamura,K.,Yonei Tamura,S.and Izpisua Belmonte,J.C.: "Molecular basis of left-right asymmetry"Development, Growth & Differentiation. 61. 645-656 (1999)

Tamura,K.、Yonei Tamura,S. 和 Izpisua Belmonte,J.C.：“左右不对称的分子基础”的发展、成长

Tsukui, T., Capdevila, J., Tamura, K., Ruiz-Lozano, P., Rodriguez-Esteban, C., Yonei-Tamura, S., Magallon, J., Chandraratna, R.A.S., Chien, K., Blumberg, B., Evans, R.M.and Izpisua Belmonte, J.C.: "Multiple left-right asymmetry defects in Shh-/-mutant mic

Tsukui, T.、Capdevila, J.、Tamura, K.、Ruiz-Lozano, P.、Rodriguez-Esteban, C.、Yonei-Tamura, S.、Magallon, J.、Chandraratna, R.A.S.、Chien, K.,