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Study on factors that determine the tissue-specific susceptibility to mouse hepatitis virus

小鼠肝炎病毒组织特异性易感性决定因素的研究

基本信息

批准号：
11680823
负责人：
KYUWA Shigeru
金额：
$ 2.43万
依托单位：
THE UNIVERSITY OF TOKYO (2000)Kumamoto University (1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680823/
关键词：
mouse hepatitis virus viral receptor strain difference replication tissue-specific 組織特異性

项目摘要

It has been speculated that viruses grow only in the specific species or tissues because many host factors are involved in viral replication cycle. Mouse hepatitis virus (MHV), which is prevalent for contemporary mouse colonies, generally infects mice and the related rodents but other mammalians. Therefore, MHV provides a good experimental model for viral infections of species-specific or tissue-specific. On the other hand, the complete understanding of MHV replication mechanism offers the possibility to produce a MHV-resistant mouse strain. Recently, viral receptor of MHV (Bgp-1), one of host factors that regulate MHV replication was cloned and has been shown to be involved in the susceptibility after intracerebral inoculation with MHV-JHM.However, MHV-JHM growth in primary culture of hepatocytes from BALB/c mice, which express Bgp-1^a that is believed susceptible genotype, was significantly higher than that from C57BL/6 mice, which also express Bgp-1^a. In contrast, MHV-JHM growth in peritoneal macrophages from BALB/c mice was comparable to that from C57BL/6 mice. There was not a significant difference between the expression of Bgp-1 in hepatocytes and macrophages from BALB/c and C57BL/6 mice. Although we tried to isolate the molecule (s) that participate in MHV-JHM replication from hepatocytes, we could not succeeded.

据推测，病毒只在特定的物种或组织中生长，因为许多宿主因素参与了病毒的复制周期。小鼠肝炎病毒（MHV），这是流行的当代小鼠群体，通常感染小鼠和相关的啮齿动物，但其他啮齿动物。因此，MHV为物种特异性或组织特异性的病毒感染提供了良好的实验模型。另一方面，对MHV复制机制的完全理解为产生MHV抗性小鼠品系提供了可能性。近年来，MHV病毒受体（Bgp-1）基因被克隆出来，并被证实与MHV-JHM脑内接种后的易感性有关。然而，MHV-JHM在原代培养的BALB/c小鼠肝细胞中的生长显著高于C57 BL/6小鼠，也表达Bgp-1 ^a。相比之下，BALB/c小鼠腹腔巨噬细胞中的MHV-JHM生长与C57 BL/6小鼠相当。BALB/c和C57 BL/6小鼠肝细胞和巨噬细胞Bgp-1的表达无显著性差异。尽管我们试图从肝细胞中分离参与MHV-JHM复制的分子，但我们未能成功。