Comprehensive studies on murine norovirus infections in mice

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Murine norovirus (MNV) is a new murine pathogen reported in2003, and there remains much to be clarified. It was found that MNV infection has spread to laboratory mouse colonies in Japan by the epidemiologic survey using serological and molecular genetical methods. To improve diagnostic methods, we developed the basis of the ELISA system using recombinant VP1 protein as antigen, and also an RT-LAMP method which was simple, speedy, and highly sensitive. Moreover, we demonstrated that hypochlorite-based disinfectants, which were commonly used in hygiene program for laboratory animal facilities in Japan, were effective in inactivation of MNV in vitro even in the presence of feces. It was shown that MNV infection modified the experimental results of dextran sulfate (DSS)-induced inflammatory bowel disease model as well as those of mouse hepatitis virus (MHV) infection model in mice.