Comprehensive risk analysis of bat-borne zoonosis with consideration to its biological features and environmental factors

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We improved the method to detect viral RNA, combining classical RDV and size segregation. We developed an IgG-ELISA system to detect antibody to Reston ebolavirus (REBOV) glycoprotein. By IgG-ELISA to glycoproteins and nuclear proteins, we examined whether 141 bat sera in Luzon Island had antibodies to REBOV or not and found that a serum of R. amplexicaudatus was positive in the antibody responses to both viral proteins. In addition, positive responses against either REBOV-GP or -NP were also observed in 15 sera of the same bat species caught in and around Mindanao Island. We characterized bat xenobiotic metabolizing enzymes, determining full-size or almost full-size nucleotide sequences of CYP1A, CYP3B, CYP2D and UGT1A.