Investigation of the mechanism in localization of atherosclerosis by computational analysis of mass transfer from flowing blood to an arterial wall based on a true-to-scale anatomical model

基于真实比例解剖模型，通过计算分析从流动血液到动脉壁的质量传递，研究动脉粥样硬化定位的机制

• 批准号: 11680831
• 负责人: WADA Shigeo
• 金额: $ 2.56万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680831/
• 关键词: Computational biomechanics; Blood flow; Mass transfer; Atherosclerosis; Intimal hyperplasia; lipoprotein; Hemodynamics; 局在化; 実形状

To elucidate the mechanism in the localization of vascular diseases such as atherosclerosis and anastomotic intimal hyperplasia in the arterial system, we carried out computational analyses of mass transport of atherogenic low density lipoproteins (LDL) from flowing blood to an arterial wall based on true-to-scale anatomical models which were obtained by animal experiments creating a mild stenosis on rabbit common carotid arteries and an end-to-side anastomotic junction on dog common carotid arteries and harvesting them at different time postoperatively. To do that, we developed an experimental system measuring the 3-dimensional configuration of a resinous cast of real artery and a new modeling method to construct anatomically a realistic model for computation from the configuration of the arterial cast. We also measured the location where intimal hyperplasia occurred and the distribution of intimal thickness in the arteries which were used to construct the computational model by microscopic observation of histological specimens prepared from the arteries. Then the relationship among the hemodynamic properties such as wall shear stress and detailed flow patterns in the vicinity of the vessel wall, LDL concentration at the luminal surface of the vessel and the thickness of the intima was investigated. As the result, it was found that LDL concentration at the luminal surface of the artery was locally elevated distal to the apex of the stenosis and at heel and toe portions of the anastomotic junction where flow was disturbed and wall shear stress was relatively low, and that such a site corresponded well to the site where intimal thickening was observed. These results suggest that blood flow plays an important role in the localized pathogenesis and development of atherosclerosis and intimal hyperplasia by affecting the degree of concentration polarization of LDL which occurs at a blood/endothelium boundary.

为了阐明动脉系统中动脉粥样硬化和吻合口内膜增生等血管疾病的定位机制，我们对致动脉粥样硬化的低密度脂蛋白（LDL）从流动的血液到动脉壁的质量运输进行了计算分析，这些模型是基于真实比例的解剖模型，这些模型是通过动物实验获得的，在兔颈总动脉上产生轻度狭窄，犬颈总动脉侧吻合口的解剖学观察，并于术后不同时间取材。为此，我们开发了一个实验系统，测量真实的动脉的三维结构和一种新的建模方法，从解剖学上构建一个现实的模型，从动脉模型的配置计算。我们还测量了内膜增生发生的位置和内膜厚度的分布在动脉中，用于构建计算模型，通过显微镜观察从动脉制备的组织学标本。然后，血流动力学特性，如壁切应力和详细的流动模式在血管壁附近，低密度脂蛋白浓度在管腔表面的血管和内膜厚度之间的关系进行了研究。结果发现，动脉管腔表面处的LDL浓度在狭窄顶点远端和吻合口的足跟和趾部局部升高，在该处血流受到干扰，壁面剪切应力相对较低，并且该部位与观察到内膜增厚的部位相对应。这些结果表明，血流通过影响血液/内皮边界处发生的低密度脂蛋白浓度极化程度，在动脉粥样硬化和内膜增生的局部发病和发展中发挥重要作用。

项目成果

岩井俊昭: "生体模擬血管内を流れる微粒子の内壁近傍における濃度分布の光学的測定"日本光学会第1回生体医用光学研究会講演論文集. 110-113 (2000)

Toshiaki Iwai：“模拟生物血管中流动的细颗粒内壁附近的浓度分布的光学测量”日本光学学会第一届生物医学光学研究会议记录110-113（2000）。

和田 成生: "粥状動脈硬化症の発症メカニズムと血流-血管壁物質輸送の計算流体力学シュミレーション"理研生命力学シミュレーション研究予稿集. 146-150 (1999)

Nario Wada：“动脉粥样硬化和血流-血管壁物质运输的发生机制的计算流体动力学模拟”理化学研究所生物力学模拟研究论文集146-150（1999）。

糀屋睦: "緩い狭窄を施した家兎総頸動脈に生じる内膜肥厚とリポ蛋白質の局所濃度との関係"信学技報. 99・138. 1-6 (1999)

Mutsumi Kojiya：“轻度狭窄的兔子颈总动脉内膜增厚与局部脂蛋白浓度的关系”IEICE 技术报告 99・138（1999）。

Shigeo Wada: "Computational study on LDL transfer from flowing blood to arterial walls"Clinical application of computational mechanics to the cardiovascular system, ed.T.Yamaguchi, Springer-Verlag, Tokyo. 157-173 (2000)

Shigeo Wada：“LDL 从流动血液转移到动脉壁的计算研究”计算力学在心血管系统中的临床应用，T.Yamaguchi 编辑，Springer-Verlag，东京。

Hidenobu Nagata: "Three-dimensional modeling of a bypass grafted artery for a CFD study"JSME Proceedings. 00-1. 263-264 (2000)

Hienobu Nagata：“用于 CFD 研究的旁路移植动脉三维建模”JSME 论文集。