Experimental and Computational Studies on Mechanical Behavior of Red Blood Cells in Blood Flow for The Development of a Hemolysis Simulator

血流中红细胞机械行为的实验和计算研究，用于开发溶血模拟器

• 批准号: 13480284
• 负责人: WADA Shigeo
• 金额: $ 9.6万
• 依托单位: Tohoku University (2002-2003)Hokkaido University (2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480284/
• 关键词: Computational Biomechanics; Blood Flow; Red blood cell; Dynamic Light Scattering; Spring Network Model; Energy principle; Particle Method; Hemolysis; レオロジー; 連銭; 形状; 変形; 動的散乱; 凝集

In order to establish the fundamental approach in the development of hemolysis simulator, we have carried out experimental and computational analyses of the mechanical behavior of elastic red blood cells (RBCs) in blood flow. In the experimental studies, we have investigated the way using dynamic light scattering to measure the deformation and aggregation of RBCs under shear flow in cone-plate rotational viscometer. It was shown that the temporal autocorrelation function on the intensity basis is the square of the sum of the autocorrelation functions on the amplitude basis of light scattering from particles and the dynamic speckle caused by the rough surface of the rotating plate in the viscometer. By using this method, the relationship between the RBC behavior in shear flow and the viscosity of the whole blood could be clarified. In the computational studies, first, we have constructed a spring network model of RBC membrane which represents the various shape change of RBC in blood flow based on the energy principle and showed that a spherical RBC transformed itself into a biconcave discoid shape, a cupped shape and a spiculate shape when the volume in decreased. Secondly, we have demonstrated the collision and aggregation of elastic RBCs in blood flow by assuming a potential functional each node of the elastic network model, which expresses the attracting and repulsive forces between neighboring membranes. Thirdly and finally, we have proposed particle method for computer simulation of bloodflow by combining the elastic RBC model and a particle model for plasma flow, and analyzed the mechanical behavior of RBCs in various blood flow. It is possible to evaluate the hemolysis from the mechanical behavior of RBC in blood flow by combining these experimental and computational methods.

为了建立在溶血模拟器的发展的基本方法，我们进行了实验和计算分析的弹性红细胞（RBC）在血液流动中的力学行为。在实验研究中，我们采用动态光散射技术，在锥板式旋转粘度计中测量了红细胞在剪切流场中的变形和聚集。结果表明，时间自相关函数的强度基础上是自相关函数的平方和的振幅基础上的光散射颗粒和动态散斑所造成的粗糙表面的旋转盘的粘度计。通过这种方法，可以阐明红细胞在剪切流中的行为与全血粘度之间的关系。在计算研究中，首先，我们根据能量原理建立了红细胞膜的弹簧网络模型，该模型描述了红细胞在血流中的各种形状变化，并表明当红细胞体积减小时，球形红细胞会转变为双凹盘状、杯状和针状。其次，我们已经证明了弹性红细胞在血液流动中的碰撞和聚集通过假设一个潜在的功能的弹性网络模型的每个节点，它表示相邻的膜之间的吸引力和排斥力。最后，将弹性红细胞模型与血浆流动的粒子模型相结合，提出了血液流动的粒子模拟方法，并分析了红细胞在不同血液流动中的力学行为。将这些实验和计算方法相结合，可以从红细胞在血流中的力学行为来评价溶血。

项目成果

K.Ishii, T.Iwai, S.Wada, M.Miyakoshi: "Simultaneous viscometry and particle sizing on the basis of dynamic light scattering"Proceedings of SPIE. 4263. 112-121 (2001)

K.Ishii、T.Iwai、S.Wada、M.Miyakoshi：“基于动态光散射的同步粘度测定和粒度测定”SPIE 论文集。

坪田健一, 和田成生, 山口隆美: "血しょう流れ場における赤血球運動の粒子法シミュレーション"日本機械学会第16回バイオエンジニアリング講演会講演論文集. 03-38. 61-62 (2004)

Kenichi Tsubota、Shigeo Wada、Takami Yamaguchi：“血浆流场中红细胞运动的粒子法模拟”日本机械工程师学会第 16 届生物工程会议论文集 03-38（2004 年）。

和田成生, 小林亮: "赤血球凝集過程の数値シミュレーション"日本機械学会講演論文集. 01-10. 297-298 (2001)

Shigeo Wada、Ryo Kobayashi：“红细胞凝集过程的数值模拟”日本机械工程师学会会议记录 01-10（2001）。

Shigeo Wada, Ryo Kobayashi: "Simulation of the shape change of a red blood cell at the entrance of a capillary"Abstract of the IV World Congress of Biomechanics. (CD-ROM). (2001)

Shigeo Wada、Ryo Kobayashi：“毛细血管入口处红细胞形状变化的模拟”第四届世界生物力学大会摘要。

K.Tsubota, S.Wada, T.Yamaguchi: "Simulation study on red blood cell motion under the flowing plasma using a particle method"Workshop on Biomechanical Engineering and Biomaterials, International Symposium on Bio-inspired System. 66-69 (2004)

K.Tsubota、S.Wada、T.Yamaguchi：“利用粒子法模拟流动血浆下红细胞运动的研究”生物力学工程与生物材料研讨会，仿生系统国际研讨会。