Investigation of non-physiological calcification in artificial organs after implantation ; OPN and macrophages

人工器官植入后非生理性钙化的调查；

• 批准号: 11680849
• 负责人: TOMIZAWA Yasuko
• 金额: $ 2.43万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680849/
• 关键词: Artificial organs; calcification; OPN; macrophage; non-physiological; blood vessel prosthesis; intima hyperplasia; migration; CD68; 動脈硬化; 生体適合性

Healing characteristics of small-caliber vascular prostheses used in small children have rarely been observed because removing specimens are troublesome. Modified Blalock Taussig shunt procedures were performed using small caliber expanded polytetrafluoroethylene (ePTFE) vascular grafts and fabric grafts in 13 patients with congenital heart disease. At the time of total corrective procedures, a piece of 10 ePTFE grafts in 10 patients and six fabric grafts in four patients were removed from the distal pulmonary anastomosis, and evaluated. The implantation duration was from 11 months to 5 years and 7 months (mean, 2 years and 6 months). At removal, average patient age was 4 years and all grafts were patent. Microscopically, the wall of three ePTFE grafts were calcified, and macrophages were immunohistologically observed in the graft wall. Thrombus formation, intimal hyperplasia, and pannus detachment was common. In the fabric grafts, many capillaries infiltrated the interstices and often reached the lumen from the perigraft side. These results suggested that in cyanotic small children, angiogenesis in and around fabric grafts was active and calcification was accelerated in ePTFE grafts. Complete endothelialization throughout the length, caused by angiogenesis, might be possible in small children when highly porous fabric vascular prostheses are used.

用于儿童的小口径人造血管的愈合特征很少被观察到，因为取出标本很麻烦。采用小口径膨体聚四氟乙烯（ePTFE）血管和织物移植物对13例先天性心脏病患者施行改良Blalock-Taussig分流术。在完全矫正手术时，从远端肺吻合口取出10例患者的10件ePTFE移植物和4例患者的6件织物移植物，并进行评价。植入时间11个月~ 5年7个月，平均2年6个月。取出时，患者平均年龄为4岁，所有移植物均通畅。显微镜下，三个ePTFE移植物的壁钙化，在移植物壁中免疫组织学观察到巨噬细胞。血栓形成、内膜增生和血管翳脱离是常见的。在织物移植物中，许多毛细血管浸润间隙，并经常从移植物周围侧到达管腔。这些结果表明，在紫绀型幼儿中，织物移植物内和周围的血管生成活跃，ePTFE移植物的钙化加速。当使用高度多孔的织物血管假体时，由血管生成引起的整个长度的完全内皮化在幼儿中可能是可能的。

项目成果

冨澤康子ほか: "心のう液中のサイトカインからみた心臓における生体反応の検討"Coronary. 16(4). 288-292 (1999)

Yasuko Tomizawa 等人：“从心液中细胞因子的角度检查心脏的生物反应”16(4) 288-292 (1999)。

冨澤康子 ほか: "人工臓器とトラッキング;医用材料植え込み患者追跡の実際"人工臓器. 29. 496-499 (2000)

Yasuko Tomizawa 等人：“人工器官和跟踪；植入医疗材料的患者的跟踪”人工器官。

Ando M, Tomizawa Y, Noishiki Y, Terada M, Imai Y: "Autologous tissue-fragmented extracardiac conduit with rapid, stable endothelialization due to angiogenesis."Jpn J Thorac Cardiovasc Surg. 48. 153 (2000)

Ando M、Tomizawa Y、Noishiki Y、Terada M、Imai Y：“自体组织碎片心外导管，由于血管生成而具有快速、稳定的内皮化。”Jpn J Thorac Cardiovasc Surg。