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Observation of reconstructing vascular wall with tissue engineering technique ; evaluation of the process of tissue remodeling

组织工程技术重建血管壁的观察；

基本信息

批准号：
14580833
负责人：
TOMIZAWA Yasuko
金额：
$ 2.37万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580833/
关键词：
tissue engineering angiogenesis wound healing scaffold REC remodeling sprout formation vessel 再生医療組織再生組織工学

项目摘要

For endothelialization of vascular grafts, capillary infiltration from adventitia side is important, since cell division is limited at the anastomotic site. Healing insufficiency exists in the process of vascular graft healing after implantation of blood vessel prosthesis in clinic. The introduction of therapeutic angiogenesis was not so successful in the area of artificial organs. The combination of cells, scaffold and growth factors may solve the problem. As the first step of reconstructing vascular wall with tissue engineering technique, the relation between scaffold and cells was evaluated in a rabbit ear chamber (REC) model. As a scaffold, cotton type atelocollagen was placed in the camber. The speed of angiogenesis was 100μm/day in the REC with scaffold and 66μm/day without scaffold. With recombinant basic fibroblast growth factor, angiogenesis accompanied by wound healing was also accelerated in rabbits. In the process of healing in the REC, angiogenesis starts with sprout formation from venous side, then become mature vessels of artery and vein. The remodeling of vessel wall was also observed. At the beginning, the vessel wall was thin, and become thick with pericytes. It is important that using tissue engineering concept of cells, extracellular matrix and growth factor in best mix style is the key for success of angiogenesis and remodeling of blood vessels.

对于血管移植物的内皮化，来自外膜侧的毛细血管浸润是重要的，因为细胞分裂在吻合部位受到限制。临床上人工血管植入后移植血管愈合过程中存在愈合不全的问题。在人造器官领域，治疗性血管生成的引入并不那么成功。细胞、支架和生长因子的结合可能解决这个问题。作为组织工程技术重建血管壁的第一步，在兔耳腔（REC）模型中评价了支架与细胞的关系。作为支架，将棉型去端胶原放置在腔中。有支架的REC血管生成速度为100μm/d，无支架的REC血管生成速度为66μm/d。用重组碱性成纤维细胞生长因子，血管生成伴随着伤口愈合也加快了在兔。在REC的愈合过程中，血管生成始于静脉侧的芽形成，然后成为动脉和静脉的成熟血管。同时观察到血管壁重塑。刚开始时，血管壁较薄，随着周细胞的出现而变厚。利用组织工程学中细胞、细胞外基质和生长因子的最佳混合方式是血管新生和血管重建成功的关键。