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Development of a vascular prosthesis with to angiogenesis

开发具有血管生成功能的血管假体

基本信息

批准号：
07671340
负责人：
TOMIZAWA Yasuko
金额：
$ 1.41万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671340/
关键词：
angiogenesis vein vascular prostheses cytokine bFGF antithrombogenicity endothelial cell blood vessel prostheses

项目摘要

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in would healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascullar prostheses and is significant fro mshortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Antithrombogenicity is important when the speed of blood is slow in the venous position for satisfactory patency. Natural and permanent antithrombogenicity due to host endothelial cells should be excellent for long-term patency. Accerelated endothelialization on the lumen of vascular prosthesis is important from shortly after implantation. We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for endothelialization. We developed a TF-graft and observed the healing process including angiogenesis and endothelialozation. Proliferation and mitosis of endothelial cells ware active in the graft wall in the arterial position 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the venous position, if we could produce stronger antithrombogenicity for a week.

碱性成纤维细胞生长因子（bFGF）是最强的血管生成剂之一，并影响参与伤口愈合的所有细胞类型。bFGF分布广泛，因为它在调节细胞增殖和有丝分裂中起重要作用。bFGF的血管生成对于血管假体的内皮化是重要的，并且在植入后不久就有意义。血管移植物壁缺乏血管生成因子可能导致新生内膜不愈合。当静脉位置的血流速度较慢时，抗血栓形成对于获得满意的通畅性很重要。由于宿主内皮细胞的天然和永久性抗血栓性对于长期通畅性应该是极好的。植入后不久，人工血管管腔上的粘附相关内皮化是重要的。我们假设组织碎片（TF-）移植物在植入后不久就具有丰富的血管生成活性，其能力可能有助于内皮化。我们研制了一种TF-移植物，并观察了愈合过程，包括血管生成和内皮化。移植后5天动脉部位移植物壁内皮细胞增殖活跃，有丝分裂活跃。在第7天，内膜的整个厚度被组织化。这些结果表明，内源性碱性成纤维细胞生长因子是重要的内皮化，由于血管生成的织物人造血管，而血栓和感染可能有负面影响。如果我们能在一周内产生更强的抗血栓性，静脉位置的移植物是可能通畅的。