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Development of hybrid vascular grafts with accelerated healing due to activated autologous cytokines

由于激活的自体细胞因子而加速愈合的混合血管移植物的开发

基本信息

批准号：
09680865
负责人：
TOMIZAWA Yasuko
金额：
$ 2.3万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in wound healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascular prostheses and is significant from shortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Collateral formation in ischemic heart disease is induced by angiogenic factors including bFGF.We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for collateral formation to the ischemic area when it is used for a small diameter vascular prosthesis in the coronary artery position. We developed a TF-graft and observed the healing process including angiogenesis and endothelialization. We designed the vascular graft which is made of fabric and tissues with cells. And, this is "Hybrid". Proliferation and mitosis of endothelial cells were active in the graft wall 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the coronary position, if we could have fine basic small-diameter fabric prosthesis with good handling characteristics, excellent antithrombogehicity.

碱性成纤维细胞生长因子（bFGF）是最强的血管生成剂之一，并且影响参与伤口愈合的所有细胞类型。bFGF分布广泛，因为它在调节细胞增殖和有丝分裂中起重要作用。bFGF的血管生成对于血管假体的内皮化是重要的，并且在植入后不久就具有重要意义。血管移植物壁缺乏血管生成因子可能导致新生内膜不愈合。缺血性心脏病侧支循环的形成是由包括bFGF在内的血管生成因子诱导的，我们假设组织碎片（TF-）移植物在植入后不久就具有丰富的血管生成活性，当其用于冠状动脉位置的小直径人造血管时，其能力可能对缺血区域的侧支循环形成起作用。我们开发了一种TF移植物并观察了愈合过程，包括血管生成和内皮化。我们设计的血管移植物是由织物和含有细胞的组织制成的。这就是“混合动力”植入后5天，移植管壁内皮细胞增殖活跃，有丝分裂活跃。在第7天，内膜的整个厚度被组织化。这些结果表明，内源性碱性成纤维细胞生长因子是重要的内皮化，由于血管生成的织物人造血管，而血栓和感染可能有负面影响。如果我们能有良好的操作特性和良好的抗血栓形成性的小直径织物假体，冠状动脉位置的移植物是可能通畅的。