Development of hybrid vascular grafts with accelerated healing due to activated autologous cytokines

碱性成纤维细胞生长因子(碱性成纤维细胞生长因子)是最强的血管生成因子之一，可影响参与伤口愈合的所有细胞类型。碱性成纤维细胞生长因子在调节细胞增殖和有丝分裂中起着重要作用，因此分布广泛。碱性成纤维细胞生长因子在血管内皮化过程中起着重要的作用，从植入后不久就开始发挥重要作用。血管移植物壁上血管生成因子的缺乏可能导致新生内膜无法愈合。在缺血性心脏病中，侧支循环的形成是由包括bFGFF在内的血管生成因子诱导的。我们假设，组织碎片(Tf-)移植物在植入后不久就具有丰富的血管生成活性，当用于冠状动脉位置的小直径血管假体时，它可能会促进侧支循环形成到缺血区。我们研制了一种转移因子移植物，并观察了愈合过程，包括血管生成和内皮化。我们设计了一种血管移植物，它是由织物和组织与细胞组成的。这就是“混血”。移植后5d，移植壁内皮细胞增殖和有丝分裂活跃。7天时内膜组织完全增厚。这些结果表明，内源性碱性成纤维细胞生长因子在织物血管假体的血管生成中起重要作用，而血栓和感染可能对血管内皮化有负面影响。如果我们能拥有良好的基本小直径织物假体，具有良好的操控性和出色的抗血栓性能，那么我们就有可能在冠状位置植入专利移植物。

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in wound healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascular prostheses and is significant from shortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Collateral formation in ischemic heart disease is induced by angiogenic factors including bFGF.We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for collateral formation to the ischemic area when it is used for a small diameter vascular prosthesis in the coronary artery position. We developed a TF-graft and observed the healing process including angiogenesis and endothelialization. We designed the vascular graft which is made of fabric and tissues with cells. And, this is "Hybrid". Proliferation and mitosis of endothelial cells were active in the graft wall 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the coronary position, if we could have fine basic small-diameter fabric prosthesis with good handling characteristics, excellent antithrombogehicity.