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Effect of laser light irradiation on channel characteristic of immunocytologic membrane.

激光照射对免疫细胞膜通道特性的影响。

基本信息

批准号：
11680857
负责人：
MITO Keiichiro
金额：
$ 1.41万
依托单位：
University of East Asia
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

In this study, the activity of the human monocytes, which are the precursor cells of macrophages, was investigated under the low power laser light irradiation (wavelength : 594 and 780 nm). The activity was measured using the radical oxygen generated from the monocytes. In the results, it was more enhanced by the irradiation of the wavelength of 780 nm than that of 594 nm. At 780nm, there were the peak of the activity at the range of 5-8 J/cm^2 and the tendency toward the low level of the activity beyond the energy range. It suggested that the activity of monocytes was enhanced depending with the wavelengths or energy levels of the laser light.On the other hand, the characteristics of the channel was investigated when the laser light irradiated on the erythrocytologic membrane at the different wavelength of 594 and 670 nm, and the different light energy. The relationships between the applied voltage and the current through the membrane was evaluated using the mono-channel method of the … More path-clamp system. In the results, the current increased linearly in proportion to the applied voltage under the red laser light irradiation of 670 nm in the range of 0 - 100 mJ/ cm^2, and there was an little increasing tendency the current at minus voltages. The resistance of the membrane was decreased in proportion to the decreasing of the voltage, and it was enhanced by the laser light irradiation. The pattern of the yellow laser light irradiation of the wavelength of 594 nm was similar to that of the red laser light irradiation. There was, however, a significant difference which was the reversed pattern of the current in the energy range of 35 - 105 mJ/ cm^2, and the pattern returned to the control state beyond the energy range. The resistance of the membrane show the minus value in all range of the applied voltages. It suggested that the membrane was stimulated and the depolarization occurred on the membrane of the cell at the energy range. There may be the threshold level of the depolarization to be changed in the depending to the wavelength or energy of the light. Less

在本研究中，研究了低功率激光照射（波长：594 和 780 nm）下人类单核细胞（巨噬细胞的前体细胞）的活性。使用单核细胞产生的自由基氧来测量活性。结果显示，780 nm 波长的照射比 594 nm 波长的照射更加增强。在780nm处，5-8J/cm^2范围内有活性峰值，超出能量范围则活性趋于低水平。这表明单核细胞的活性增强取决于激光的波长或能量水平。另一方面，研究了当激光以594和670 nm的不同波长和不同的光能量照射在红细胞膜上时的通道特性。使用路径钳系统的单通道方法评估施加的电压和通过膜的电流之间的关系。结果表明，在670 nm红色激光照射下，在0～100 mJ/ cm^2范围内，电流与外加电压成正比线性增加，负电压下电流略有增加趋势。膜的电阻随着电压的降低成比例地降低，并且通过激光照射而增强。波长594nm的黄色激光照射的图案与红色激光照射的图案类似。然而，有一个显着的差异，即在 35 - 105 mJ/ cm^2 的能量范围内电流的模式发生逆转，并且在超出能量范围时电流模式返回到控制状态。膜的电阻在所有施加电压范围内均显示负值。表明在该能量范围内细胞膜受到刺激，细胞膜发生去极化。消偏振的阈值水平可以根据光的波长或能量而改变。较少的