Project study of tissue regeneration with tissue engineering

组织工程组织再生项目研究

• 批准号: 12307051
• 负责人: YAMADA Satoru
• 金额: $ 25.43万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307051/
• 关键词: tissue regeneration; tissue engineering; Proliferating tissue; human periodontal ligament; gene profiling; bFGF; SPARC; neurotrophin; 遺伝子治療; mRNA; CPC; PLLA; 臨床系; 組織再構築; rhBMP-2; エナメル基質タンパク; CO2レーザー

1. Mesenchymal stem cells : Proliferating tissue in periodontal osseous defects promotes the formation of new periodontal tissue around the root(Yamada.Ota). We find that the extracellular matrix is a critical regulator of the induction of alkaline phosphatase and the formastion of multiple layered in human gingival fibroblast(Maeda). One clone, PDL-29, identified as a COX assembly factor, showed much stronger m RNA expression in HPEs than in HGFs in culture. (Takashiba)2. Signaling molecules :Placement of PGS between the root surface and rhBMO-2 3 PGS complex had the effect to decrease ankylosis(Kawanami). SPARC plays arole in repair of periodontal tissues by promotingproliferation and osteoclastogenesis inhibitory factor production(Kurihara). EMDOGAIN has a potential to promote the cementum regeneration and to create a favorable environment that will promote the periodontal regeneration. CPC seemed to act as a scaffold for bone formation and histocompatible healing of periodontal tis … More sues(Oda). PGE2, cAMP-elevating agent, EP2/EP4 agonist stimulated cAMP accumulation in HPDL cells. However, BMP-2 hand no effect on it, and per-treatment with BMP-2 also did not cause significant changes in the cAMP accumulation stimulated with PGE2 or EP2 / EP4 agonist(Hasegawa). LJE becomes shorter, whereas the proliferative activity of regenerative connective tissue maintains the same level of proliferation, and ultimately LJE is replaced by regenerative connective tissue(Hashimoto9.Topical application of 0.1-0.4% bFGF induced significant periodontal tissue regeneration in animal experiments. In vitro studies demonstrated that bFGF stimulation in the presence of fetal calf serum inhibited proliferation of gingival epithelial cells and induced the release of hyaluronan and heparan sulfate from periodontal ligament cells (Murakami)3. Scaffolds : The PLLA membrane showed an excellent results in comparison with the PLGA membrane in vitro experiment. Moreover, the histological observation showed no different bone regeneration in dogs in between PLLA and PLGA membrane(Izumi). The effect of matrix geometry upon the periodontal reconstruction induced by BMP was studied. This study using geometrically different cell substrata demonstrated that a matrix with a certain geometrical size is most favorable for cell differentiation((Takida) Less

1.间充质干细胞：牙周骨缺损中的再生组织促进牙根周围新牙周组织的形成（山田.大田）。我们发现细胞外基质是诱导碱性磷酸酶和人牙龈成纤维细胞（Maeda）多层结构形成的关键调节因子。一个克隆PDL-29被鉴定为考克斯装配因子，在培养的HPE中比在HGF中表现出更强的mRNA表达。2.信号分子：在牙根表面和rhBMO-2 3 PGS复合物之间放置PGS具有减少关节强直的作用（Kawanami）。牙周炎是通过促进破骨细胞生成抑制因子（Kurihara）的产生和增殖来修复牙周组织的。EMDOGAIN具有促进牙骨质再生的潜力，并创造了一个有利的环境，将促进牙周再生。CPC似乎是一种骨形成和牙周组织相容性愈合的支架材料 ...更多信息 小田（Oda）。PGE_2、cAMP升高剂、EP_2/EP_4激动剂可促进HPDL细胞内cAMP的积累。然而，BMP-2对其没有影响，并且用BMP-2预处理也没有引起用PGE 2或EP 2/EP 4激动剂（Hasegawa）刺激的cAMP积累的显著变化。LJE变得更短，而再生结缔组织的增殖活性保持相同的增殖水平，最终LJE被再生结缔组织取代（Hashimoto 9.在动物实验中局部应用0.1- 0.4%bFGF诱导显著的牙周组织再生。体外研究表明，在胎牛血清的存在下，bFGF刺激抑制牙龈上皮细胞的增殖，并诱导牙周韧带细胞释放透明质酸和硫酸乙酰肝素（Murakami）3。支架材料：PLLA膜在体外实验中表现出优异的结果。此外，组织学观察显示PLLA和PLGA膜（Izumi）在犬中的骨再生没有差异。研究基质几何形状对BMP诱导牙周组织重建的影响。这项研究使用几何形状不同的细胞基质证明，具有一定几何尺寸的基质最有利于细胞分化（（Takida）Less

项目成果

石川烈, 小田茂, 小島丈尚: "永末書店"周組織再生とエナメルタンパク. 149 (2002)

Retsu Ishikawa、Shigeru Oda、Takehisa Kojima：“Eisue Shoten”周围组织再生和牙釉质蛋白 149 (2002)。

Shimono M. et.al.: "Connexins in salivary glands"European Journal of Morphology. 38・4. 257-261 (2000)

Shimono M.等人：“唾液腺中的连接蛋白”欧洲形态学杂志38・4（2000）。

Fujimoto C, Maeda H, Kokeguchi S, Takashiba S, Nishimura F, Arai H, Fukui K, Murayama Y: "Application of denaturing gradient gel electrophoresis(DGGE) to the analysis of microbial communities of subgingival plaque."Journal of Periodontal Research. 38(4).

Fujimoto C、Maeda H、Kokeguchi S、Takashiba S、Nishimura F、Arai H、Fukui K、Murayama Y：“变性梯度凝胶电泳 (DGGE) 在龈下菌斑微生物群落分析中的应用。”牙周研究杂志。

Shimono M, Ishikawa T, Ishikawa H, Matsuzaki H, Hashimoto S, Muramatsu T, Shima K, Inoue T.: "Regulatory mechanisms of periodontal regeneration."Microsc Res Tech.. 60(5). 491-502 (2003)

Shimono M、Ishikawa T、Ishikawa H、Matsuzaki H、Hashimoto S、Muramatsu T、Shima K、Inoue T.：“牙周再生的调节机制”。Microsc Res Tech.. 60(5)。

Usuda J., Hashimoto S., Enokiya Y., Inoue T., Shimono M.: "Proliferative activities of epithelial and connective tissue cells in the rat periodontal regeneration using AgNORs staining."J.Periodont.Res.. (in printing). (2004)

Usuda J.、Hashimoto S.、Enokiya Y.、Inoue T.、Shimono M.：“使用 AgNORs 染色观察大鼠牙周再生中上皮和结缔组织细胞的增殖活性。”J.Periodont.Res..（印刷中）