Project study of tissue regeneration with tissue engineering

1. 间充质干细胞：牙周骨缺损中的增殖组织促进牙根周围新牙周组织的形成（Yamada.Ota）。我们发现细胞外基质是人牙龈成纤维细胞诱导碱性磷酸酶和多层形成的关键调节剂（Maeda）。其中一个克隆PDL-29被鉴定为COX组装因子，在培养的HPEs中显示出比hgf更强的m RNA表达。(Takashiba) 2。信号分子：在根表面和rhBMO-2 - 3 PGS复合物之间放置PGS具有减轻强直的作用（Kawanami）。SPARC通过促进增殖和破骨细胞生成抑制因子的产生在牙周组织修复中发挥作用（Kurihara）。EMDOGAIN具有促进牙骨质再生和创造有利于牙周再生的环境的潜力。CPC似乎可以作为牙周炎骨形成和组织相容性愈合的支架…More sues（Oda）。PGE2、cAMP升高剂、EP2/EP4激动剂刺激HPDL细胞cAMP积累。然而，BMP-2对其没有影响，并且BMP-2预处理也没有引起PGE2或EP2 / EP4激动剂刺激的cAMP积累的显着变化（Hasegawa）。LJE变短，而再生结缔组织的增殖活性保持相同的增殖水平，最终LJE被再生结缔组织所取代(Hashimoto9。局部应用0.1-0.4% bFGF可诱导显著的牙周组织再生。体外研究表明，胎牛血清刺激bFGF可抑制牙龈上皮细胞增殖，并诱导牙周韧带细胞释放透明质酸和硫酸肝素（Murakami）3。支架：在体外实验中，与PLGA膜相比，PLLA膜表现出良好的效果。此外，组织学观察显示，PLLA和PLGA膜对狗的骨再生没有影响（Izumi）。研究基质几何形态对BMP诱导牙周重建的影响。该研究使用几何形状不同的细胞基质，表明具有一定几何尺寸的基质最有利于细胞分化（(Takida) Less）

1. Mesenchymal stem cells : Proliferating tissue in periodontal osseous defects promotes the formation of new periodontal tissue around the root(Yamada.Ota). We find that the extracellular matrix is a critical regulator of the induction of alkaline phosphatase and the formastion of multiple layered in human gingival fibroblast(Maeda). One clone, PDL-29, identified as a COX assembly factor, showed much stronger m RNA expression in HPEs than in HGFs in culture. (Takashiba)2. Signaling molecules :Placement of PGS between the root surface and rhBMO-2 3 PGS complex had the effect to decrease ankylosis(Kawanami). SPARC plays arole in repair of periodontal tissues by promotingproliferation and osteoclastogenesis inhibitory factor production(Kurihara). EMDOGAIN has a potential to promote the cementum regeneration and to create a favorable environment that will promote the periodontal regeneration. CPC seemed to act as a scaffold for bone formation and histocompatible healing of periodontal tis … More sues(Oda). PGE2, cAMP-elevating agent, EP2/EP4 agonist stimulated cAMP accumulation in HPDL cells. However, BMP-2 hand no effect on it, and per-treatment with BMP-2 also did not cause significant changes in the cAMP accumulation stimulated with PGE2 or EP2 / EP4 agonist(Hasegawa). LJE becomes shorter, whereas the proliferative activity of regenerative connective tissue maintains the same level of proliferation, and ultimately LJE is replaced by regenerative connective tissue(Hashimoto9.Topical application of 0.1-0.4% bFGF induced significant periodontal tissue regeneration in animal experiments. In vitro studies demonstrated that bFGF stimulation in the presence of fetal calf serum inhibited proliferation of gingival epithelial cells and induced the release of hyaluronan and heparan sulfate from periodontal ligament cells (Murakami)3. Scaffolds : The PLLA membrane showed an excellent results in comparison with the PLGA membrane in vitro experiment. Moreover, the histological observation showed no different bone regeneration in dogs in between PLLA and PLGA membrane(Izumi). The effect of matrix geometry upon the periodontal reconstruction induced by BMP was studied. This study using geometrically different cell substrata demonstrated that a matrix with a certain geometrical size is most favorable for cell differentiation((Takida) Less