The Mechanisms of Plaque-induced Lesion of Peri-implantitis

牙菌斑诱发种植体周围炎病变的机制

• 批准号: 08457511
• 负责人: YAMADA Satoru
• 金额: $ 3.14万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457511/
• 关键词: implant; plaque; peri-implantitis; pathology; dog; インプラト; インプラント周囲炎; Branemark; Integral; 細菌叢; 組織検索; 細菌

The aim of the present study was to investigate histpathologically the tissue destruction of pen-implant and periodontium due to subgingival plaque which was accumulated by ligature. The mandibular premolars of eight mongrel dogs were extracted. Eight titanium implant fixtures were installed after three months. Plaque control was maintained on the implants as well as adjacent premolars during one month prior to the start of the experiment. Ligatures were placed on the subgingival portion of eight implants and the adjacent teeth to promote plaque rormation for three months. Tissues of pen-implant and periodontium were dissected, embedded in paraffin and examined morphometrically and immunohistochemically. The extention of inflammation, the location of attached epithelium and the position of the alveolar bone crest were measured morphometnically. Antibody CD45Ro was employed as a T-cell marker, and CD2Ocy was used as a B-cell marker. Infiltration of inflammatory cells into the apical portion were more remarkable in the pen-implant tissue than the gingival tissue. Considerable bone loss was detected around the implants, but not the control teeth. There was no significant difference in the proportion of T and B lymphocytes in the inflammatory lesion of both pen-implant and natural teeth. These results suggesed that inflammation spreads deeper and more rapidly in the pen-implant than natural teeth.

本研究的目的是从组织病理学的角度探讨结扎引起的龈下菌斑对笔式种植体和牙周组织的破坏。拔除8只杂种犬的下颌前磨牙。三个月后安装了八个钛种植体固定装置。在实验开始前的一个月内，在种植体以及相邻的前磨牙上保持菌斑控制。在8个种植体的龈下部分和邻近牙齿上放置结扎物，以促进菌斑形成，为期3个月。将笔式种植体和牙周组织解剖，石蜡包埋，并进行形态计量学和免疫化学检查。对炎症范围、附着上皮的位置和牙槽骨嵴的位置进行形态计量学测量。抗体CD45Ro用作T细胞标志物，CD20cy用作B细胞标志物。在笔式种植体组织中，炎性细胞向根尖部的浸润比牙龈组织更显著。在种植体周围检测到相当大的骨丢失，但对照牙齿没有。笔式种植体与天然牙炎症病变中T、B淋巴细胞的比例无显著性差异。这些结果表明，炎症在笔式种植体中比天然牙齿传播得更深、更快。

项目成果

関口一実: "プラーク蓄積下のおけるイヌの顎骨内インテグラル・インプラント周囲組織の経時的変化" 歯科学報. 95. 1241- (1995)

Kazumi Sekiguchi：“牙菌斑堆积下狗颌骨整体种植体周围组织的时间变化”《牙科杂志》95. 1241-（1995）。

穂坂康朗: "プラークのイヌインプラント周囲組織に及ぼす影響について" 日歯周誌. 38. 339-345 (1996)

Yasuaki Hosaka：“牙菌斑对犬种植体周围组织的影响”日本牙周病学杂志 38. 339-345 (1996)。

山之内 一也: "イヌのインプラント周囲組織にプラークが及ぼす影響に関する組織的研究" 日本歯周病学会会誌. 38・4. 457-464 (1996)

Kazuya Yamanouchi：“牙菌斑对狗种植体周围组织影响的系统研究”日本牙周病学会杂志 38・4（1996）。

MIKIO OTA: "HISTOPATHOLOGICAL STUDY OF PLAQUE-INDUCED LESIONS OF PERI-IMPLANT AND PERIODONTAL TISSUES IN DOGS" J.Japan.Soc.Periodont. 41・1. 16-27 (1999)

MIKIO OTA：“狗种植体周围和牙周组织斑块引起的病变的组织病理学研究”J.Japan.Soc.Periodont 16-27 (1999)。

ATUSHI SAITO: "PESPONESES OF PERI-IMPLANT TISSUES TO UNDISTURBED PLAQUE FORMATION IN DOGS : CLINICAL,RADIOGRAPHIC,AND MICROBIOLOGICAL FINDINGS" Bull.Tokyo Dnt.Coll.38・1. 13-20 (1997)

ATUSHI SAITO：“狗植入物周围组织对未受干扰斑块形成的影响：临床、放射学和微生物学发现”Bull.Tokyo Dnt.Coll.38・1 (1997)。