The Mechanisms of Plaque-induced Lesion of Peri-implantitis

本研究的目的是从组织病理学的角度研究结扎术对种植体和牙周组织的破坏作用，以探讨结扎术对种植体和牙周组织的影响。拔除8只杂种犬的下颌前磨牙。3个月后安装8个钛种植体固定物。在实验开始前的一个月内，对种植体和相邻的前磨牙进行菌斑控制。在8颗种植体的龈下部分和相邻的牙齿上放置结扎物，以促进菌斑形成，为期3个月。切取种植体和牙周组织，石蜡包埋，进行形态计量学和免疫组织化学检测。用形态计量学方法测量炎症程度、附着上皮的位置和牙槽骨的位置。以CD45Ro抗体作为T细胞标志物，CD2Ocy作为B细胞标志物。种植体周围组织中炎性细胞在根尖部位的渗入较牙龈组织更为明显。种植体周围有大量的骨丢失，但对照牙没有。种植体牙周炎性病变中T、B淋巴细胞比例与天然牙相比无显著差异。这些结果表明，炎症在笔状种植体中的扩散比天然牙更深、更快。

The aim of the present study was to investigate histpathologically the tissue destruction of pen-implant and periodontium due to subgingival plaque which was accumulated by ligature. The mandibular premolars of eight mongrel dogs were extracted. Eight titanium implant fixtures were installed after three months. Plaque control was maintained on the implants as well as adjacent premolars during one month prior to the start of the experiment. Ligatures were placed on the subgingival portion of eight implants and the adjacent teeth to promote plaque rormation for three months. Tissues of pen-implant and periodontium were dissected, embedded in paraffin and examined morphometrically and immunohistochemically. The extention of inflammation, the location of attached epithelium and the position of the alveolar bone crest were measured morphometnically. Antibody CD45Ro was employed as a T-cell marker, and CD2Ocy was used as a B-cell marker. Infiltration of inflammatory cells into the apical portion were more remarkable in the pen-implant tissue than the gingival tissue. Considerable bone loss was detected around the implants, but not the control teeth. There was no significant difference in the proportion of T and B lymphocytes in the inflammatory lesion of both pen-implant and natural teeth. These results suggesed that inflammation spreads deeper and more rapidly in the pen-implant than natural teeth.