Analysis of the Mechanisms for Soilborne Plant Disease Development using REMI-Mutants of Fusarium oxysporum
利用尖孢镰刀菌 REMI 突变体分析土传植物病害发展机制
基本信息
- 批准号:12660051
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
About 20 pathogenicity mutants of Fusarium oxysporum ff. sp. conglutinans (cabbage yellows pathogen ; FOC) and lycopersici (tomato wilt pathogen ; FOL) were selected from about 2500 transformants generated by restriction enzyme-mediated integration (REMI) mutagenesis.REMI10, a pathogenicity-deficient mutant of FOC, was proved that a copy of pCNS43 was inserted into the genome. The gene disrupted by the insertion of pCSN43 was rescued and the gene seemed to encode an aspartic proteinase. We designated the gene fap1. fapl disruptants of FOC carried pathogenicity to cabbage equilibrant with FOC, showed that FAP1 is not responsible to FOC for pathogenicity. Pretreatment of cabbage root with REMI10 reduced the disease incidence of yellows caused by FOC. This suggested that REMI 10 carried biocontrol activity. Behavior of green fluorescence protein (GFP)-expressing REMI10 (EGFP-REMI10) was observed under fluorescence microscope and we could see that EGFP-REMI10 penetrate into cabbage root through cuticle slower than FOC and EGFP-REMI10 did not go through the endodermis to the xylem even 14 days after inoculation.r-120, a reduced pathogenicity mutant of FOL, was also proved that a copy of pCNS43 was inserted into the genome. The gene tagged in the mutant with pCSN43 was predicted to encode a protein of 321 amino acids and designated foir1, Homology search showed its partial similarity to a chloride conductance regulatory protein of Xenopus laevis, suggesting that FOIR1 is a transmembrane protein. Although the role of FOIR1 has never been identified, it may participate to the pathogenicity in FOL, because FOIR1 deficient mutants of FOL reproduced reduced pathogenicity on tomato.
大约20个尖孢镰刀菌的致病突变体。SP.从2500个转化子中筛选出粘着菌(甘蓝黄化病原菌FOC)和番茄枯萎病病原菌(FOL),证明FOC的致病力缺失突变体REMI10已将pCNS43插入基因组。被pCSN43插入中断的基因被挽救,该基因似乎编码了一种天冬氨酸蛋白酶。我们将该基因命名为fap1。FOC的FAPL干扰物对甘蓝的致病力与FOC平衡,表明FAP1对FOC的致病性不起作用。用REMI10处理甘蓝根部,可降低FOC引起的黄化发病率。这表明Remi 10具有生防活性。在荧光显微镜下观察了表达绿色荧光蛋白(GFP)的REMI10(EGFP-REMI10)的行为,发现EGFP-REMI10通过角质层进入甘蓝根部的速度慢于FOC,即使在接种后14d,EGFP-REMI10也没有通过内胚层进入木质部。用pCSN43标记的基因编码321个氨基酸的蛋白,命名为foir1,同源性搜索表明它与非洲爪哇的氯离子电导调节蛋白部分相似,提示FOIR1是一种跨膜蛋白。虽然FOIR1的作用尚未确定,但它可能参与了FOL的致病作用,因为FOIR1基因缺失的突变体对番茄的致病力降低。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Saitoh Ki: "Targeted gene dosruption of the neuronal calcium sensor 1 homologue in rice blast fungus, Magnaporthe grisea"Biosci Biotechnol Biochem. 67(3). 651-653 (2003)
Saitoh Ki:“稻瘟病菌 Magnaporthe grisea 中神经元钙传感器 1 同源物的靶向基因破坏”Biosci Biotechnol Biochem。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshida, T., Miyata, Y., Kawabe, M., Kadota, L, Tsuchiya, K., Teraoka. T. and Arie. T.: "A pathogenicity-deficient mutant of Fusarium oxysporum f. sp. conglutinans, REMI10 - Its biocontrol activity and analysis of the tagged gene"Mol. Plant-Microbe Intera
吉田,T.,宫田,Y.,川部,M.,角田,L,土屋,K.,寺冈。
- DOI:
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- 影响因子:0
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Fukiya S: "Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea"Biosci Biotechnol Biochem. 66(3). 663-666 (2002)
Fukiya S:“稻瘟病菌 Magnaporthe grisea 中推定的液泡丝氨酸蛋白酶基因的鉴定”Biosci Biotechnol Biochem。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Arie, T.: "Trends in Biocontrol Research on Soilborne Plant Diseases: In Integrated Management Programs on Clubroot Disease of Cruciferous. in Agrochemical Discovery: insect. weed. and fungal control"American Chemical Society. 317(142-151) (2001)
Arie, T.:“土传植物病害生物防治研究趋势:十字花科根肿病综合管理计划。农业化学发现:昆虫、杂草和真菌控制”美国化学会。
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- 影响因子:0
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- 通讯作者:
Kamakura T: "A novel gene, CBP1, encoding a putative extracellular chitin-binding protein, may play an important role in the hydrophobic surface sensing of Magnaporthe grisea during appressorium differentiation"Mol Plant Microbe Interact. 15・5. 437-444 (2
Kamakura T:“一种编码假定的细胞外几丁质结合蛋白的新基因 CBP1,可能在附着胞分化过程中稻瘟病菌的疏水表面感应中发挥重要作用”Mol Plant Microbe Interact. 15・5.
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ARIE Tsutomu其他文献
ARIE Tsutomu的其他文献
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{{ truncateString('ARIE Tsutomu', 18)}}的其他基金
Elucidation of the evolution and function of the accessory chromosomes in ascomycete fungi and their application asnovel transformation vector
子囊菌副染色体进化和功能的阐明及其作为新型转化载体的应用
- 批准号:
19H00939 - 财政年份:2019
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Study on mechanisms of pathogenicity divergence in Fusarium oxysporum by structural and functional analyses of small chromosomes
小染色体结构与功能分析尖孢镰刀菌致病力趋异机制研究
- 批准号:
16H02536 - 财政年份:2016
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Use of Pyricularia oryzae, the rice blast fungus, as a bio microsyringe to inject protein biopesticide into plant tissue
使用稻瘟菌(稻瘟病菌)作为生物微注射器将蛋白质生物农药注入植物组织
- 批准号:
26660040 - 财政年份:2014
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Analyses of function, structure, and origin of the avirulence determining genomic locus in the tomato wilt fungus, Fusarium oxysporum f. sp. Lycopersici
番茄枯萎病真菌 Fusarium oxysporum f 中决定无毒力的基因组位点的功能、结构和起源分析。
- 批准号:
18380030 - 财政年份:2006
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of the evolutionary history of phytopathogenic fungi of tomato based on gene genealogies
基于基因谱系的番茄植物病原真菌进化史分析
- 批准号:
16405021 - 财政年份:2004
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Control of soilborne disease by foliar spray of plant activators and analysis of its mechanisms
植物活化剂叶面喷施防治土传病害及其机理分析
- 批准号:
15380033 - 财政年份:2003
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of Evolutionary History of Phytopathogenic Fungi based upon Evidence from DNA Genealogies
基于 DNA 谱系证据的植物病原真菌进化史分析
- 批准号:
14405024 - 财政年份:2002
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Asexuality in Fusarium oxysporum: Mechanisms and Relationship between Pathogenicity-Diversity
尖孢镰刀菌的无性生殖:致病性与多样性之间的机制及关系
- 批准号:
10660053 - 财政年份:1998
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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马铃薯天冬氨酸蛋白酶的结构-功能关系
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