Asexuality in Fusarium oxysporum: Mechanisms and Relationship between Pathogenicity-Diversity

尖孢镰刀菌的无性生殖：致病性与多样性之间的机制及关系

• 批准号: 10660053
• 负责人: ARIE Tsutomu
• 金额: $ 2.3万
• 依托单位: RIKEN
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660053/
• 关键词: Fusarium oxysporum; Alternaria alternata; ascomycetes; mating type gene (MAT); asexual; gene expression; RT-PCR; heterologous expression; PCR; 萎凋病菌; ポリガラクツロナーゼ; 系統樹; 進化

Mating type (MAT) loci were cloned from asexual ascomycetes Fusarium oxysporum and Alternaria alternata by PCR-based strategy. Structure of the MAT loci in the genome of F. oxysporum was compared to that of Gibberella fujikuroi, a sexual relative of F. oxysporum. MAT1-1 idiomorph encodes three genes (Mat1-1-1, MAT1-1-2, and MAT1-1-3) which were highly homologous between the two species; MAT1-2 idiomorph encodes single MAT1-2-1 gene that was also homologous between the species. All of the MAT genes were confirmed to be expressed in asexual F. oxysporum when it was cultured on MM medium as well as in the sexual G. fujikuroi. A. alternata MAT genes (MAT1-1-1 and MAT1-2-1) were also expressed on MM medium and moreover both genes were proved to be functional by heterologous expression in Cochliobolus heterostrophus. These results suggested that the cause of asexuality in ascomycetes does not depend on MAT loci.

利用聚合酶链式反应技术，从无性子囊菌、尖孢镰刀菌和交链格孢菌中克隆了交配型(MAT)基因座。比较了尖孢镰刀菌基因组mat基因座与尖孢镰刀菌的有性亲缘关系藤库里赤霉菌基因组中的mat基因座的结构。MAT1-1基因编码三个基因(MAT1-1-1、MAT1-1-2和MAT1-1-3)，MAT1-2基因编码单个MAT1-2-1基因，这三个基因在两个物种之间高度同源。经MM培养，所有MAT基因均在无性尖孢霉和有性藤冈孢霉中表达。互隔交链孢霉的MAT基因(MAT1-1-1和MAT1-2-1)也在MM培养基上表达，并且这两个基因在Cochliobolus herostrophus中的异源表达被证明是有功能的。这些结果表明，子囊菌无性性的原因不依赖于MAT基因座。

项目成果

Kawabe, M.: "Assessment of mating-type of Gibberella fujikuroi by PCR."Ann. Phytopatol. Soc. Jpn.. 65 (3). 342-343 (1999)

Kawabe, M.：“通过 PCR 评估藤黑赤霉的交配型。”Ann。

有江力: "子のう菌の交配型遺伝子"日本農薬学会誌. 25・1. 44-50 (2000)

Riki Arie：“子囊菌的交配型基因”日本农药学会杂志25・1（2000）。

T. Arie: "Assessment of Gibberella fujikuori mationg type by PCR"Mycoscience. 40・3. 311-314 (1999)

T. Arie：“通过 PCR 评估赤霉交配型”Mycoscience 40・314（1999）。

川部眞登: "Gibberella fujikuroi交配型のPCR法による識別"日本植物病理学会報. 65・3. 342-343 (1999)

川部正人：“通过PCR方法鉴定藤黑赤霉交配型”日本植物病理学会会刊65・3 342-343（1999）。

有江 力: "Fusarium oxysporumのポリガラクツロナーゼ遺伝子ベースの系統樹と交配型に関する考察" 日本植物病理学会報. 64・4. 373-373 (1998)

Tsutomu Arie：“基于多聚半乳糖醛酸酶基因的尖镰孢交配类型的讨论”日本植物病理学会杂志64・4（1998）。