Control of soilborne disease by foliar spray of plant activators and analysis of its mechanisms

植物活化剂叶面喷施防治土传病害及其机理分析

• 批准号: 15380033
• 负责人: ARIE Tsutomu
• 金额: $ 10.18万
• 依托单位: National University Corporation Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380033/
• 关键词: plant activator; tomato; soilborne diseases; induced resistance; foliar spray; disease control; mechanisms; SAR; バナナ; バリダマイシンA; トマト萎凋病; GFP

Tomato wilt, caused by the soilborne fungus Fusarium oxysporum f.sp.lycopersici, is effectively controlled by a foliar spray of validamycin A (VMA) or validoxylamine A (VAA)(【greater than or equal】10 μg/ml) ; however, neither VMA nor VAA is antifungal in vitro. In pot tests, the effect of a foliar application of 100 μg/ml VMA or VAA lasted for 64 days. Plants sprayed with VMA or VAA accumulated salicylic acid, and had elevated expression of the systemic acquired resistance (SAR) marker genes P4(PR-1), Tag (PR-2), and NP24(PR-5). Foliar spray of VMA also controlled late blight and powdery mildew of tomato. The disease control by VMA and VAA lasted up to 64 days after treatment, was broad spectrum and induced the expression of PR genes, all essential indicators of SAR, suggesting that VMA and VAA are plant activators. The foliar application of plant activators is a novel control method for soilborne diseases and may provide an economically feasible alternative to soil fumigants such as methyl bromide.

由土传真菌尖孢镰刀菌番茄专化型引起的番茄枯萎病可通过叶面喷洒井冈霉素A（VMA）或井冈羟胺A（VAA）（[大于或等于]10 μg/ml）有效防治;然而，VMA和VAA在体外均不具有抗真菌性。盆栽试验中，叶面喷施100 μg/ml的VMA或VAA，效果持续64天。用VMA或VAA喷洒的植物积累水杨酸，并且具有系统获得抗性（SAR）标记基因P4（PR-1）、Tag（PR-2）和NP 24（PR-5）的升高的表达。叶面喷施VMA对番茄晚疫病和白粉病也有一定的防治效果。VMA和VAA的病害控制持续长达64天的治疗后，是广谱的，并诱导PR基因的表达，所有的SAR的基本指标，表明VMA和VAA是植物激活剂。植物活化剂的叶面施用是一种新的控制土传病害的方法，并可能提供一种经济可行的替代土壤熏蒸剂，如甲基溴。

项目成果

バリダマイシンAの茎葉散布によるトマト萎凋病防除効果のメカニズム

井冈霉素A叶面喷施防治番茄枯萎病的作用机制

• 发表时间: 2006
• 期刊: 植物防疫 60・4
• 作者: 石川亮;有江 力
• 通讯作者: 有江 力

Induction of resistance against rice bacterial leaf blight by 3-chloro-1-methyl-1H-pyrazole-5-carboxylic acid

• DOI: 10.1584/jpestics.30.47
• 发表时间: 2005-01-01
• 期刊: JOURNAL OF PESTICIDE SCIENCE
• 影响因子: 2.4
• 作者: Nishioka, M;Nakashita, H;Yamaguchi, I
• 通讯作者: Yamaguchi, I

Foliar spray of validamycin A or validoxylamine A controls tomato Fusarium wilt

• DOI: 10.1094/phyto-95-1209
• 发表时间: 2005-10-01
• 期刊: PHYTOPATHOLOGY
• 影响因子: 3.2
• 作者: Ishikawa, R;Shirouzu, K;Arie, T
• 通讯作者: Arie, T

プラントアクチベーターのスクリーニング⇒

植物活化剂筛选⇒

• 发表时间: 2006

"3 Fungicides". in Pesticide Science (eds Sato K, et al.)

“3种杀菌剂”。

• 发表时间: 2003
• 作者: Yasuda;M.;Nakashita H;Yasuda M;Arie T;有江 力;Arie T
• 通讯作者: Arie T