Analisis of molecular mechanism of new signal transeduction that regurates sexal development in Schizosaccharomyces pombe.

在裂殖酵母中，细胞分化到减数分裂的分子机制已经被广泛研究，启动交配需要整合与某些环境线索(如氮饥饿)和交配伙伴S(信息素)的存在有关的信号信息。然而，环境刺激是如何感知的，以及它们是如何与细胞分化途径的激活联系在一起的，目前还知之甚少。在这项研究中，我们对组氨酸激酶(Phk1，Phk2，Phk3)和转录因子Prr1进行了鉴定，发现它们在序列发育的调节中起着至关重要的作用。我们发现，氧化应激信号不仅是诱导性发育的重要参数。His-to-Asp磷酸传递途径是由三个组氨酸激酶(Phk1/2/3)以及Prr1和Mcs4对氧化胁迫的反应组成的，与有氧和厌氧对S.pombe的生长有重要的生理联系。

In the fission yeast Schizosaccharomyces pombe, the molecular mechanisms underlying the cell differentiation into meiosis have been extensively characterized and initiate mating requires the integration of signal information concerning both certain environmental cues (e. g., nitrogen starvation) and the the presence of mating partner s(pheromones). However, it is pooriy understood how environmental stimuli are perccivcd, and then how they are linked to the activation of the cell differentiation pathways. In this study, we characterized the histidine kinases(Phk1, Phk2, Phk3) and the transcription factor Prr1, and they were reveaied that they play a crucial role in regulation of sezual development. We found that not also an oxidative stress signal is a crucial paramenter for induction of sexual development in S. pombe. The avidence are presented for that the His-to-Asp phosphorelay pathway, composed of the hree histidine kinases (Phk1/2/3) and the Prr1 and Mcs4 response to oxidative stress, with significant physiological relevance to aerobiosis and anaerobiosis for growth in S. pombe.