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Neurogenesis of Photoreceptor Cells in the Pineal Organ and Retina of the Adult Fish

成鱼松果体和视网膜感光细胞的神经发生

基本信息

批准号：
17580162
负责人：
OMURA Yuri
金额：
$ 2.46万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17580162/
关键词：
adult fish pineal organ retina photoreceptor neurogenesis PCNA BrdU rhodopsin immunocytochemistry

项目摘要

Cell proliferation in the pineal organ of the immature and matured rainbow trout Oncorhynchus mykiss was investigated by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA) and BrdU (thymidine analogue) -labeling together with photoreceptor-specific opsin. Numerous PCNA-immunoreactive cells were found throughout the pineal end-vesicle and stalk. Two types of PCNA-immunoreactive cells were distinguished : intensely stained, large ovoid and round cells, and mildly stained, slender fusiform cells. Occasionally, close approaches were found between the opsin-immunoreactive photoreceptor outer segments and the PCNA-immunoreactive cells, which expressed mildly stained, nuclear and cytoplasmic signals. In addition, close connections or overlaps of the opsin-immunoreactive outer segments with the BrdU-labeled cells were found here and there within the pineal epithelium of the rainbow trout. Compared with these findings of the rainbow trout, PCNA-immunoreactive cells or BrdU-labeled cells were found scarcely in the pineal organs of the other fish, such as tilapia Oreochromis niloticus, zebrafish Danio rerio and goldfish Carassius auratus. Consequently, these results suggest that the proliferation and neurogenesis of the pineal photoreceptor cells persist through the life of the rainbow trout, thus maintaining highly sensitive, photo-signal transduction mechanisms for melatonin synthesis and reproduction

用增殖细胞核抗原(增殖细胞核抗原)和BrdU(胸腺嘧啶核苷类似物)标记的免疫细胞化学方法，结合光感受器特异性视蛋白，研究了未成熟和成熟虹鱼松果体的细胞增殖。在松果体末端囊泡和柄中可见大量的增殖细胞核抗原免疫反应阳性细胞。增殖细胞核抗原免疫反应阳性细胞可区分为两种类型：染色较深的大卵圆形细胞和染色较淡的细长梭形细胞。偶见视蛋白免疫反应阳性的光感受器外段与表达轻度染色、胞核和胞浆信号的增殖细胞核抗原免疫反应细胞之间的近距离。此外，在虹鱼松果体上皮内，视蛋白免疫反应阳性的外节与BrdU标记的细胞之间也有紧密的联系或重叠。与虹鱼相比，其他鱼类如罗非鱼、斑马鱼和金鱼的松果体中几乎没有发现增殖细胞核抗原免疫反应细胞或BrdU标记细胞。因此，这些结果表明，松果体光感受器细胞的增殖和神经发生持续了彩虹鲑鱼的一生，从而维持了褪黑素合成和繁殖的高度敏感的光信号转导机制。