Development of a novel enzymatic synthesis method for trehalose derivatives and its application

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We aimed at the development of novel enzymatic synthesis method of the trehalose derivatives and those applications to insecticides or anti-fungal chemicals. Trehalose is a disaccharide and commonly found in fungi, algae and in several invertebrate phyla but not in flowering plants and vertebrates. It presents in all insects and in many insects it is present in high concetration s and constitutes the major hemolymph sugar. Trehalose secifically hydrolyzed trehalose and is the rate-limiting enzyme in the process of glycogen synthesis from hemolymph trehalose in developing ovaries of the silkworm. Therefore the specific inhibitor for trehalases is a candidate of novel insecticides.We purified glucose-3-dehydrogenase from Halomonas sp. A -15 and found its activity to produce trehalose derivatives. One of those derivatives, 3,3'-diketotrehalose showed an inhibitory activity to trehalose, and therefore we investigated its activity as insecticide or anti-fungal chemicals. It did not show anti-fungal activity but it can be an insecticide. We also investigated its efficient production process, and found the enzymatic production is more efficient than microbial production. By using potassium ferricyanide as electron acceptor and the highly purified glucose-3-dehydrogenase, 80% productivity was attained. Hence we established efficient method to produce 3,3'-diketotrehalose, it can contribute as a novel effective insecticide which is not harmful to animals.