The Development of Interface Molecules for Enzyme Electrochemical Reaction
酶电化学反应界面分子的研究进展
基本信息
- 批准号:14350431
- 负责人:
- 金额:$ 9.09万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In most of oxidoreductases, the prosthetic group is buried deeply and within the protein shell. Thus a direct electrochemical recycling of the enzyme's prosthetic group at the electrode surface leading to a corresponding current signal is rarely encountered. In order to improve enzyme sensor signal property, we focused on the application of electron transfer proteins, cytochromes as the molecular interface between oxidorcductase redox center and electrode surface. In nature, various dehydrogenases harboring either PQQ or FAD are coupled with the respiratory chain via cytochromes. Based on this electron transfer property, we considered the utilization of cytochrome as the interface molecule to facilitate the electron transfer between enzyme and the artificial electron mediator or the electrode in the enzyme based biosensor. In this project, we focused on the application and engineering of cytochrome b_<562> as the interface molecule for novel bio-electronic devices and processes employi … More ng various oxidoreductases. The impact of the presence of cytochrome b_<562> on the various oxidoreductases was investigated, and its properties as the interface molecule were confirmed, by observing improved electrochemical response in the sensors employing these combinations. The engineering of cytochrome b_<562> was then attempted. The improvement of affinity toward PQQ glucose dehydrogenase was achieved by either modifying its surface charge or by introducing novel bio-affinity ligands. The employment of engineered interface molecules resulted in the bioelectrochemical reaction with PQQGDH, consequently improved the sensor properties. Finally, the co-expression of PQQ glucose dehydrogenase and cytochromeb_<562> was attempted to construct novel process employing interface molecules. The co-expression of PQQ glucose dehydrogenase and cytochromeb_<562> resulted in the construction of engineered E.coli cell which was able to transfer electron directly with electrode in the absence of artificial synthetic electron mediators. The results suggested the construction of novel bio-electrochemical process employing in vivo expressing interface molecules. Less
在大多数氧化还原酶中,辅基深埋在蛋白质壳内。因此,在电极表面的酶的辅基的直接电化学再循环导致相应的电流信号很少遇到。为了改善酶传感器的信号特性,我们重点研究了电子传递蛋白、细胞色素作为氧化还原酶氧化还原中心与电极表面之间的分子界面的应用。在自然界中,携带PQQ或FAD的各种酶通过细胞色素与呼吸链偶联。基于这种电子传递特性,我们考虑利用细胞色素作为界面分子,促进酶与人工电子介质或电极之间的电子传递,在酶基生物传感器。在本项目中,我们重点研究了细胞色素B_<562>作为新型生物电子器件和工艺的界面分子的应用和工程。 ...更多信息 ng各种氧化还原酶。细胞色素B_的存在下<562>对各种氧化还原酶的影响进行了研究,并确认其作为界面分子的性质,通过观察改善电化学响应的传感器采用这些组合。然后尝试了细胞色素B_的工程化<562>。通过修饰PQQ葡萄糖脱氢酶的表面电荷或引入新的生物亲和配体,实现了对PQQ葡萄糖脱氢酶的亲和力的改善。工程化界面分子的应用导致了与PQQGDH的生物电化学反应,从而改善了传感器的性能。最后,我们尝试将PQQ葡萄糖脱氢酶与细胞色素b_2共表达<562>,构建界面分子的新工艺。将PQQ葡萄糖脱氢酶和细胞色素b_2共表达<562>,构建了能够在不需要人工合成电子介质的情况下直接用电极传递电子的工程大肠杆菌细胞。结果表明,构建新的生物电化学过程中使用在体内表达的界面分子。少
项目成果
期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Multi-sugar Analysis System Using Novel Glucose 3-Dehydrogenase Electrode
使用新型葡萄糖 3-脱氢酶电极的多糖分析系统
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:T.Hamafuji;Y.Takano;W.Tsugawa;K.Sode
- 通讯作者:K.Sode
Akane Sakaguchi, Wakako Tsugawa, Stefano Ferri, Koji Sode: "Development of highly-sensitive fructosyl-valine enzyme sensor employing recombinant fructosyl amine oxidase"Electrochemistry. 71(6). 442-445 (2003)
Akane Sakaguchi、Wakako Tsukawa、Stefano Ferri、Koji Sode:“采用重组果糖基胺氧化酶开发高灵敏度果糖基缬氨酸酶传感器”电化学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Glucose enzyme electrode using cytochrome b_<562> as electron mediator
使用细胞色素b_<562>作为电子介体的葡萄糖酶电极
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Junko Okuda;Junko Wakai;Noriko Yuhashi;Koji Sode
- 通讯作者:Koji Sode
Engineered PQQ Glucose Dehydrogenase-based Enzyme Sensor for Continuous Glucose Monitoring
基于 PQQ 葡萄糖脱氢酶的工程酶传感器,用于连续血糖监测
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Junko Okuda;Junko Wakai;Satoshi Igarashi;Koji Sode
- 通讯作者:Koji Sode
Junko Okuda, Junko Wakai, Noriko Yuhashi, Koji Sode: "Glucose enzyme electrode using cytochrome b_<562> as electron me mediator"Biosensors and Bioelectronics. 18. 699-704 (2003)
Junko Okuda、Junko Wakai、Noriko Yuhashi、Koji Sode:“使用细胞色素 b_<562> 作为电子介质的葡萄糖酶电极”生物传感器和生物电子学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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SODE Koji其他文献
SODE Koji的其他文献
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{{ truncateString('SODE Koji', 18)}}的其他基金
Structural regulation of amyloid forming proteins with pyrroloquinoline quinone
吡咯喹啉醌对淀粉样蛋白形成蛋白的结构调节
- 批准号:
20360369 - 财政年份:2008
- 资助金额:
$ 9.09万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a novel enzymatic synthesis method for trehalose derivatives and its application
海藻糖衍生物酶法合成新方法的建立及其应用
- 批准号:
12839003 - 财政年份:2000
- 资助金额:
$ 9.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Artificial symbiosis based bio-process
基于人工共生的生物过程
- 批准号:
09650868 - 财政年份:1997
- 资助金额:
$ 9.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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