Synaptische Transmission zwischen Mitralzelle und Körperzelle im olfaktorischen Bulbus der Ratte: Zwei-Photonen-Mikroskopie der synaptischen Calciumströme und simultane Elektrophysiologie in vitro

大鼠嗅球二尖瓣细胞和体细胞之间的突触传递：突触钙电流的双光子显微镜和体外同步电生理学

• 批准号: 5309314
• 负责人: Professorin Dr. Veronica Egger
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Emmy Noether International Fellowships
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2003-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5309314?language=en
• 关键词: Synaptische; Transmission; zwischen; Mitralzelle; und

The proposed project aims at a better understanding of early olfactory processing, in which the large dendrodendritic reciprocal synapse between mitral cells and granule cells is a main player. Our approach will be based on simultaneous application of two-photon imaging of calcium dynamics and conventional electrophysiology of synaptic transmission in vitro. Given a long-term goal of understanding the nature of processing of olfactory information in the bulb at a systemic level, the following lines of research are proposed: Having investigated the calcium dynamics following stimulation of the mitral cell-granule cell synapse, we will measure the influence of the emotion- and arousal-related neuromodulator norepinephrine on calcium transients and synaptic transmission in this synapse, since there are strong neuromodulatory projections into the olfactory bulb. Finally, we plan to investigate transmission at the mitral cell-granule cell reciprocal synapse in the context of a more intact circuit, in order to further elucidate the influence of centrifugal inputs on signal processing in the olfactory bulb. To do so, we will develop an "integrative" slice preparation containing also extrabulbar elements, i.e. part of the olfactory cortex.

该项目旨在更好地理解早期嗅觉处理，其中二尖瓣细胞和颗粒细胞之间的大树突突触是主要参与者。我们的方法将基于钙动力学双光子成像和体外突触传递传统电生理学的同时应用。鉴于在系统水平上了解球中嗅觉信息处理本质的长期目标，提出了以下研究方向：在研究了刺激二尖瓣细胞-颗粒细胞突触后的钙动力学后，我们将测量情绪和唤醒相关的神经调节剂去甲肾上腺素对该突触中钙瞬变和突触传递的影响，因为存在强烈的神经调节投射 进入嗅球。最后，我们计划在更完整的电路背景下研究二尖瓣细胞-颗粒细胞相互突触的传递，以进一步阐明离心输入对嗅球信号处理的影响。为此，我们将开发一种“综合”切片制剂，其中还包含球外元素，即嗅觉皮层的一部分。