权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Physiological and biochemical studies on freezability boar spermatozoa

公猪精子冷冻性的生理生化研究

基本信息

批准号：
12660256
负责人：
TERADA Takato
金额：
$ 2.3万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The effects of exposure to 2-hydroxyprophl-beta-cyclodextrin(HBCD) and methyl-beta-cyclodextrin(MBCD) on the freezability and cold shocked sensitivity of boar spermatozoa were investigated in the first experiment. The motility, progressive motility and velocity of either cold-shocked or frozen-thawed spermatozoa in cyclodextrin exposure treatments were significantly higher than those of the control. Preloading cyclodextrin with cholesterol-3 sulfate resulted in a decrease in all aforementioned criteria. These observations demonstrate that the artificial modification of its membrane improves the resistance of boar spermatozoa to cold shock and cryopreservation stress. We next examined whether the exposure of spermatozoa to seminal plasma before freezing decreases its freezability, assessed by %motile cells and in vitro penetration ability using IVF and chlortetracycline fluorescence assessment. When subjected to cryopreservation, the spermatozoa incubated in seminal plasma before freezi … More ng showed significantly lower levels of post-thaw motility than did spermatozoa incubated in modified Hulsenberg VIII diluents. The incubation of spermatozoa in seminal plasma also significantly prevented frozen-thawed spermatozoa from penetrating the oocytes. The next experiment, using unfrozen spermatozoa, was to determine if the incubation of spermatozoa with seminal plasma reduced the penetration ability before freezing, resulting in a significantly lower penetration rate after freezing compared with spermatozoa incubated without seminal plasma. The penetration competence of unfrozen spermatozoa was also found to be significantly decreased by incubation in seminal plasma, but to difference in motility was observed between the treatments with seminal plasma and modified Hulsenberg VIII diluents. It was concluded that ejaculated seminal plasma contains some factor(s) that modified the sperm before freezing, negatively influence the freezability and penetration competence of spermatozoa after freezing. Less

实验一研究了2-羟丙基-β-环糊精（HBCD）和甲基-β-环糊精（MBCD）对猪精子冷冻性和冷休克敏感性的影响。环糊精处理组的精子活力、前向运动能力和运动速度均显著高于对照组。用胆固醇-3硫酸酯预加载环糊精导致所有上述标准的降低。这些结果表明，人工修饰猪精子膜可提高猪精子对冷休克和冷冻应激的抵抗力。我们接下来检查了冷冻前精子暴露于精浆是否会降低其冷冻性，通过使用IVF和金霉素荧光评估的运动细胞%和体外穿透能力进行评估。冷冻保存时，冷冻前在精浆中孵育的精子， ...更多信息 ng显示出显着较低水平的解冻后的活力比精子孵育在修改后的Hulsenberg VIII稀释液。精子在精浆中的孵育也显著阻止了冻融精子穿透卵母细胞。下一个实验，使用未冷冻的精子，是为了确定精子与精浆孵育是否降低了冷冻前的穿透能力，导致冷冻后的穿透率显著低于无精浆孵育的精子。未冷冻精子的穿透能力也被发现通过在精浆中孵育而显著降低，但在用精浆和改良的Hulsenberg VIII稀释液处理之间观察到运动性的差异。结果表明，精浆中含有某些因子，这些因子在冷冻前对精子有修饰作用，对冷冻后精子的冷冻性和穿透能力有负面影响。少