Identification of pathogenic genes in very virulent herpesviruses by analysis on single-nucleotide polymorphism.

通过单核苷酸多态性分析鉴定强毒力疱疹病毒的致病基因。

基本信息

批准号：
12660289
负责人：
ENDOH Daiji
金额：
$ 1.41万
依托单位：
Rakuno Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660289/
关键词：
subtraction herpesvirus representational difference analysis Marek's disease virus preparation of genome bovine herpesvirus 1 porcine herpesvirus 1 equine herpesvirus 1

项目摘要

Although some vaccines are in use, new superior vaccines should be developed. On the development of vaccines, construction of non-virulent strain by destruction of virulent genes of virulent strains is basic strategy. But virulent genes have not been determined in many virulent viruses. Virulent genes are determined by referring interstrain polymorphism of genome between virulent and non-virulent strains.In this study, we tried to determine virulent genes of very virulent herpesviruses using single-nucleotide polymorphisms (SNPs) as interstrain polymorphism markers, which is in the spotlight in human genomic research. On searching SNPs, preparation of viral fragments, which is suitable for detecting SNPs, is presumed to be most important step for detecting SNPs. Although many methods for detection of SNPs have been reported, method for rapid preparation of many viral DNA fragments have not been reported as a step for detection of SNPs. Thus, we focused on development of the method for preparation of many types of viral DNA fragments and also determination of appropriate size of the fragments to detect SNPs. Resultantly, we got the results below and achieved the first object of this research.Firstly, we developed a method for preparation of vial DNA fragments from avian and mammalian herpesviruses independent on its genomic sequences. Secondly, we counted SNPs between virulent and non-virulent strains of herpesviruses and determined the sizes appropriate for determination of SNPs.

尽管正在使用一些疫苗，但应开发新的上级疫苗。关于疫苗的开发，通过破坏有毒菌株的有毒基因来构建非毒性菌株是基本策略。但是在许多毒性病毒中尚未确定有毒基因。强烈的基因是通过将毒性和非毒性菌株之间基因组的界面间多态性确定的。在这项研究中，我们试图使用单核苷酸多态性（SNP）确定非常毒性疱疹病毒的毒性基因，将其视为人体间多态性标志物，在人类基因组研究中占有焦点。在搜索SNP时，假定适合检测SNP的病毒片段的制备是检测SNP的最重要步骤。尽管已经报道了许多用于检测SNP的方法，但尚未报告许多病毒DNA片段的快速制备方法作为检测SNP的一步。因此，我们专注于制备许多类型的病毒DNA片段的方法，并确定片段的适当大小以检测SNP。结果，我们得到了下面的结果，并实现了这项研究的第一个对象。首先，我们开发了一种方法来制备来自鸟类和哺乳动物疱疹病毒的基因组序列的小瓶DNA片段。其次，我们对疱疹病毒的毒和非毒性菌株进行了计算，并确定了适合确定SNP的尺寸。