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Effects of radiation on expression of NT-3 gene or right-left asymmetrical development related genes

辐射对NT-3基因或左右不对称发育相关基因表达的影响

基本信息

批准号：
12670034
负责人：
SUMIDA Hiroshi
金额：
$ 1.73万
依托单位：
Hiroshima International University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

The effects of high energy X-ray to White Leghorn embryos, or ^<252>Cf or 3 MeV neutron to mouse embryos on morphogenesis were studied. In order to check effects of radiation on neural crest cells, expression of a NT-3 gene was checked. Also, expression of a Pitx2 gene regarding to the right-left asymmetrical development was checked.As the results, the right and left axis of a embryo was reversed by neutron irradiation. Decreasing of NT-3 gene expression by high energy X-ray was recognized. The degree of decreasing of gene expression was different in the embryos. Expression of the Pitx2 gene was also decreased after irradiation. Also, the cases that suggests mutation of the Pitx2 gene was recognized. It was indicated that expression of certain genes such as NT-3 were especially affected by radiation. Certain homeobox genes, however, were not mutated by radiation.Besides, expression of genes regarding to the cell defense to radiation was studied. I examined effects of high energy X-ray to chicken embryos on expression of p53, ATM and RAD51. After 24 hr of 5 Gy or 8 Gy irradiation, expression of p53 and ATM gene was decreased significantly compared with controls. P53 and ATM are the important gene regarding to G_1 arrest and cell death by radiation irradiation, thus the results suggested that radiation affected cell defense system. Expression of RAD51 genes was increased to 2 times with controls after radiation irradiation. This result is different from a previous reports, thus, it is suggested that radiation irradiation activates DNA recombinant repair system.

研究了高能X射线对White Leghorn胚胎的影响，或252 Cf或3 MeV中子对小鼠胚胎对形态发生的影响。为了检查辐射对神经嵴细胞的影响，检查了NT-3基因的表达。另外，还检查了与左右不对称发育有关的Pitx2基因的表达。结果，通过中子照射，胚胎的左右轴颠倒了。认识到高能X射线可降低NT-3基因表达。胚胎中基因表达降低的程度不同。辐射后 Pitx2 基因的表达也下降。此外，还发现了表明Pitx2基因突变的病例。结果表明，某些基因（例如 NT-3）的表达尤其受辐射影响。然而，某些同源盒基因并未因辐射而突变。此外，还研究了与细胞对辐射的防御有关的基因的表达。我检查了高能 X 射线照射鸡胚胎对 p53、ATM 和 RAD51 表达的影响。 5 Gy或8 Gy照射24小时后，p53和ATM基因的表达与对照相比显着降低。 P53和ATM是与放射线照射引起的G_1阻滞和细胞死亡有关的重要基因，因此结果表明放射线影响了细胞防御系统。放射线照射后，RAD51 基因的表达增加至对照的 2 倍。这一结果与之前的报道不同，因此表明放射线照射激活了DNA重组修复系统。