权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular physiology of TRP channels induced via activation of metabotropic receptors

通过代谢型受体激活诱导的 TRP 通道的分子生理学

基本信息

批准号：
12670052
负责人：
MORI Yasuo
金额：
$ 2.05万
依托单位：
Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Cellular stimulation from the surrounding extracellular environment via mechanisms including plasma membrane receptors induces activation of Ca^<2+>-permeable cation channels that form essential signaling pathways in controlling biological responses. An imp ortant clue to understand the molecular mechanisms underlying these cation channels (termed as receptor-activated cation channels (RACC)) was only provided through studies of the transient receptor potential (trp) protein (TRP), which controls light-induced cationic currents in Drosophila photoreceptor cells. By using the genetic information and recombinant expression technique, numerous mammalian TRP homologues have been discovered. This further lead to identification of novel RACC_s. In the session, I would like to introduce our contribution to the recent dramatic progress in investigating mammalian RACC_s, revealing 1) critical involvement of TRP1 in both nyinduced Ca^<2+> release and store-operated Ca2+ entry, 2) identification of TRP6 as α_1-adrenergic receptor-activated cation channels in smooth muscle cells, and 3) essential interaction between redoxsy stem and TRP-related channel activation. Thus, TRP-related channels are revealed to be a powerful tool in understanding important physiological responses and for the invention of novel pharmaceutical targets.

通过包括质膜受体在内的机制，来自周围细胞外环境的细胞刺激诱导Ca^2+-可渗透阳离子通道的活化，所述阳离子通道形成控制生物反应的基本信号传导途径。瞬时受体电位（transmittance receptor potential，trp）蛋白（transient receptor potential protein，TRP）是果蝇感光细胞中控制光诱导阳离子电流的蛋白质，它的研究为理解这些阳离子通道（receptor-activated cationchannels，RACC）的分子机制提供了重要线索。利用遗传信息和重组表达技术，已经发现了许多哺乳动物TRP同源物。这进一步导致鉴定新的RACC_s。在这一部分中，我将介绍我们在哺乳动物RACC_s研究中取得的巨大进展，揭示了1）TRP 1在NY诱导的Ca^2+释放和钙库操纵的Ca 2+内流中的重要作用，2）TRP 6作为α_1-肾上腺素能受体激活的平滑肌细胞阳离子通道的鉴定，3）氧化还原系统与TRP相关通道激活之间的重要相互作用。因此，TRP相关通道被揭示为理解重要生理反应和发明新的药物靶点的有力工具。