Studies on the bioactive substances produced by plerocercoids of Spirometra erinaceieuropaei

刺猬螺旋体产生生物活性物质的研究

• 批准号: 12670231
• 负责人: HIRAI Kazumitsu
• 金额: $ 2.37万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670231/
• 关键词: Spirometra erinaceieuropaei; Plasmodium falciparum-cultured supernatant; Macrophage; TNF-a; IL-1b; Cyclooxygenase; Bioactive substances; 免疫抑制; lL-1β; MAPKカスケード; 擬充尾虫; 生理活性物質; 成長因子; TNF-α抑制物質

(1) The excretory/secretory (ES) products from plerocercoids of Spirometra erinaceieuropaei suppressed the TNF-α mRNA expression and production in by reducing phosphorylation of ERK1/2 p38MAPK without any effects on NF-kB activity in LPS-stimulated murine macrophages.(2) The ES products also suppressed IL-1β and cyclooxygenase-2 mRNA expressions by reducing phosphorylation of ERK1/2 and p38 MAPK in LPS-stimulated murine macrophages.(3) The ES product from plerocercoids of Spirometra erinaceieuropaei suppressed the TNF-α and IL-1β but not ICAM-1 mRNA expression in human monocytes, THP-1 cells stimulated with the Plasmodium-cultured supernatant.In light of these results, ES products appear to suppress gene expressions of cytokines by reducing phosphorylation of MAPK pathway in macrophage family cells. Therefore, we will try to investigate the effect of ES products on the osteoclast cells.

(1)在lps刺激的小鼠巨噬细胞中，ERK1/2 p38MAPK的磷酸化降低了TNF-α mRNA的表达和产生，而对NF-kB活性没有影响。(2) ES产物还通过降低lps刺激小鼠巨噬细胞ERK1/2和p38 MAPK的磷酸化来抑制IL-1β和环氧化酶-2 mRNA的表达。(3)经疟原虫培养的上清液刺激的人单核细胞THP-1细胞中，羊角螺粒ES能抑制TNF-α和IL-1β的表达，但不能抑制ICAM-1 mRNA的表达。根据这些结果，ES产品似乎通过降低巨噬细胞家族细胞中MAPK通路的磷酸化来抑制细胞因子的基因表达。因此，我们将尝试研究ES产品对破骨细胞的影响。

项目成果

パラマサリ・デイルガハュ: "Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress the TNF-α gene expression by reducing phosphorylation of ERK1/2 and p38MAPK in macrophages"International Journal for Parasitology. 32(9). 1155-1162 (200

Paramasari Deirgaha：“来自 Spirometra erinaceieuropaei 的排泄物/分泌产物通过减少巨噬细胞中 ERK1/2 和 p38MAPK 的磷酸化来抑制 TNF-α 基因表达”国际寄生虫学杂志 32(9)。

三浦憲豊: "Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress gene expression nad production of tumor necrosis factor-a in murine macrophages stimulated with lipopolysaccaride or lipoteichoic acid."Int.J.Parasitol.. 31(1).

Noritoyo Miura：“来自 Spirometra erinaceieuropaei 的 plerocercoids 的排泄/分泌产物抑制用脂多糖或脂磷壁酸刺激的小鼠巨噬细胞中的基因表达和肿瘤坏死因子 -a 的产生。”Int.J.Parasitol.. 31(1)。

Miura K: "Excretory/secretory products from plerocercoids of Spirometra erimaceieuropaeisuppress gene expressions and production of Tumor necrosis factor-a in murine macrophages stimulated with lipopolysaccharide or lipoteichoic acid"International Journal

Miura K：“来自 Spirometra erimaceieuropaei 的 plerocercoids 的排泄/分泌产物抑制脂多糖或脂磷壁酸刺激的小鼠巨噬细胞中基因表达和肿瘤坏死因子-a 的产生”国际期刊

パラマサリ・デイルガハユ: "Excretory/secretory products from plerocerooids of Spirometra erinaoeieuropaei suppresses the TNF-α gene expression by reducing phosphorylation of ERK1/2 and p38 MAPK in macrophages"International Journal of Parasitology. 32(9). 1155-1162 (2

Paramasari Deirgahayu：“Spirometra erinaoieuropaei 的排泄/分泌产物通过减少巨噬细胞中 ERK1/2 和 p38 MAPK 的磷酸化来抑制 TNF-α 基因表达”《国际寄生虫学杂志》32(9)。

Parmasari Dirgahayu et al.: "Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress the TNF-a gene expression by reducing phosphorylation of ERK1/2 and p38 MAPK in macrophages"International Journal for Parasitology. 32(9).

Parmasari Dirgahayu 等人：“来自 Spirometra erinaceieuropaei 的 plerocercoids 的排泄/分泌产物通过减少巨噬细胞中 ERK1/2 和 p38 MAPK 的磷酸化来抑制 TNF-a 基因表达”国际寄生虫学杂志。