Studies on the Growth Hormone-Like Factor Produced by Plerocercoids of Spirometra erinacei.

猴头螺旋体产生类生长激素因子的研究。

• 批准号: 63570175
• 负责人: HIRAI Kazumitsu
• 金额: $ 1.34万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570175/
• 关键词: Growth hormone-like substance; Spirometra erinacei; Plerocercoid; Growth factor; Hepatocyte growth factor; Growth hormone receptor; 成長ホルモン様物質; 成長ホルモン; 初代培養肝細胞増殖活性; ヒト成長ホルモンモノクロナ-ル抗体; 成長促進; 肝細胞初代培養; 成長ホルモン受容体アフィニティクロマトグラフィ

In order to examine the physiological property and partial amino acid sequence of the Growth Hormone (GH) -like factor produced by plerocercoids of Spirometra erinacei, we tried to purify this factor detected bt radio-receptor assay for GH using the GH receptor-coupled affinity chromatography. The GH-like factor were purified.1) One of GH-like factor was purified by two steps consisted of the affinity chromatography and gel filtration.2) The specific activity in this GH-like factor was enhanced to 195 times compared with the specific activity in the crude worm extract.3) This factor exhibited the high affinity for the GH receptor and its molecular weight was estimated to be approximately 16 KD by SDS-polyacryl amide gel electrophoresis.4) This factor stimulated the prolifelation of the mouse hepatocyte in vitro, and then suppressed the serum level of glucose when this factor was injected into the femoral vein of a golden hamster compared with the control.5) This 16 KD protein obviously cross-reacted with anti-human GH monoclonal antibody on the immunoblot method.6) The 27 KD protein, which had low affinity for the GH receptor, was also purified from the worm extract and cross-reacted with anti-human GH-monoclonal antibody. Moreover, this stimulated the proliferation of the mouse hepatocyte in vitro.7) There was the homology of 67% between amino acid sequences of cathepsin L and the fragment of the 27 KD protein cleavaged by lysil endo peptidase.

为了研究刺猬迭宫绦虫裂头蚴产生的生长激素（GH）样因子的生理特性和部分氨基酸序列，采用GH受体偶联亲和层析法对该因子进行了纯化。通过亲和层析和凝胶过滤两步纯化获得了一种GH样因子。2）该因子的比活性比虫体粗提物提高了195倍。3）该因子对GH受体具有很高的亲和力，SDS-聚丙烯酰胺凝胶电泳测得其分子量约为16 KD。4）该因子在体外刺激小鼠肝细胞增殖，并将其注入金黄地鼠的股静脉后，与对照组相比，抑制血清葡萄糖水平。5）免疫印迹法显示该16 KD蛋白与抗人GH单克隆抗体有明显的交叉反应。6）27 KD蛋白，对GH受体的亲和力低，也从蠕虫提取物中纯化，并与抗人GH-单克隆抗体交叉反应。7）组织蛋白酶L的氨基酸序列与Lysil内切肽酶裂解的27 KD蛋白片段的氨基酸序列同源性为67%。

项目成果

坪井 敬文他: "マンソン裂頭条虫擬充尾虫によるマウス肝細胞増殖促進作用" 寄生虫学雑誌. 38. 87- (1989)

Takafumi Tsuboi 等人：“曼氏梭菌对小鼠肝细胞增殖的促进作用”，寄生虫学杂志 38. 87- (1989)。

Hirai, K. et al.: "Growth factor induced by plerocercoids of Spirometra erinacei-Physiological activity of Sparganum Growth Factor." Igaku no Ayumi. 146(2). 836 (1988)

Hirai, K. 等人：“由猴头螺的 plerocercoids 诱导的生长因子 - 三棱生长因子的生理活性。”

平井 和光: "マンソン裂頭条虫擬充尾虫由来の成長促進因子ーSparganum Growth Factorの生理作用." 医学のあゆみ. 146. 836 (1988)

Kazumitsu Hirai：“三棱生长因子的生理作用，一种源自曼氏三棱的生长促进因子。” 医学史 146. 836 (1988)。

坪井 敬文他: "マンソン裂頭条虫擬充尾虫由来の肝細胞増殖因子" 寄生虫学雑誌. 39. 84- (1990)

Takafumi Tsuboi 等人：“源自 C. mansoni、Ta. mansoni 的肝细胞生长因子”，寄生虫学杂志 39. 84- (1990)。

Tsuboi,T.et al.: "Decrease of liver glycogen content in golden hamsters infected with plerocercoids of Spirometra erinacei." Parasitology Research. (1990)

Tsuboi,T.et al.：“感染了猴头菇的金仓鼠肝糖原含量降低。”