Identification or cellular factors associated with cytomegalovirus replication by cDNA microarrays

人巨细胞病毒（HCMV）在正常人成纤维细胞中有效复制，但在大多数永生化细胞系中复制受限或几乎不复制。为了研究HCMV征服和控制允许成纤维细胞进行后续病毒繁殖的分子策略，我们使用具有代表NCBI RefSeq数据库中9，600个人类命名基因的合成多核苷酸的微阵列系统分析了HCMV感染细胞的转录组。我们编译了从Wile型HCMV感染的和UV灭活的HCMV感染的成纤维细胞以及用感染细胞的培养基接种的成纤维细胞获得的表达谱，并发现HCMV通过吸附和/或穿透病毒颗粒以及随后通过初始事件后分泌的细胞外因子引起广泛的细胞反应。此外，野生型和紫外线灭活的病毒感染的细胞之间的转录组的比较显示，HCMV最终抑制细胞基因的表达，这是负责通过在病毒复制周期从头合成的病毒基因产物对外源性刺激。这些结果表明，主要参与炎症的细胞责任基因的抑制可能表明宿主细胞对病毒的总体服从。

Human cytomegalovirus (HCMV) replicates efficiently in normal human fibroblasts but restrictedly or scarcely in most immortalized cell lines. To investigate a molecular strategy under which HCMV conquests and governs the permissive fibroblasts toward succeeding virus propagation, we analyzed transcriptomes for HCMV-infected cells using a microarray system with synthetic polynucleotides representing 9,600 of human named genes in the RefSeq database of NCBI. We compiled expression profiles obtained from wile-type HCMV-ingected and UV-inactivated HCMV-infected fibroblasts as well as fibroblasts inoculated with culture media of the infected cells and found that HCMV elicits a wide range of cellular responses via adsorption and/or penetration of viral particles and subsequently via factors exrtracellularly secreted after the initial events. Moreover, comparison of the transcriptomes between wild type and UV-inactivated virus-infected cells revealed that HCMV eventually suppresses expression of the cellular genes which are responsible against exogenous stimuli by means of viral gene products synthesized de novo during the viral replication cycle. These results suggest that suppression of the cellular responsible genes mostly involved in inflammation may indicate the overall submission to of the host cell to the virus.