Experimental study of effects of vitamin K2 for prevention of osteoporosis.

维生素K2预防骨质疏松症作用的实验研究。

• 批准号: 12670372
• 负责人: KATSUYAMA Hironobu
• 金额: $ 1.15万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670372/
• 关键词: vitamin K_2; osteocalcin; RANK; RANKL; osteoprotegerin; real time PCR; SSH; RT-PCR

Previous study showed that nuclear binding proteins to vitamin K2 were found in osteoblast. In order to clarify the action of vitamin K2 on osteoblastic function, various concentrations of vitamin K 2 were administered to MC3T3E1 cells, which originated in mouse osteoblasts. Concentrations of 10 7M to 10 5M of vitamin K2 were administered to MC3T3E1 cells not only vitamin K2 alone but also with 10 8M of activated vitamin D. In cell growth, numbers of cells administered various vitamins were higher than that of control cells. Therefore, we investigated the effects サof vitamin K2 on osteocalcin mRNA, receptor activator of NFkB CNK) mRNA, RANK ligand (RANKL) mRNA and osteoprotegerin mRNA. These mRNA levels were increased at 1 hour and 6 after vitamin K2 administration at concentration of 10 6M. Same tendency was observed in addition of vitamin D. Since vitamin K2 seemed to affect cell function, suppression subtractive hybridization method (S SH) were used to detect the factors which might affect cell function. In this method, we detected the genes such as tenascin, lumican, etc. Further investigation is needed to detect the genes which is affected by vitamin K2.

以往的研究表明，在成骨细胞中发现了维生素K2的核结合蛋白。为了阐明维生素K2对成骨细胞功能的作用，将不同浓度的维生素K2给予来源于小鼠成骨细胞的MC3T3E1细胞。将浓度为10 7M至10 5M的维生素K2不仅单独给予MC3T3E1细胞，而且与10 8M的活化维生素D一起给予MC3T3E1细胞。在细胞生长中，给予各种维生素的细胞数量高于对照细胞。因此，我们研究了维生素K2对骨钙素mRNA、NF κ B受体激活剂（CNK）mRNA、RANK配体（RANKL）mRNA和骨保护素mRNA的影响。这些mRNA水平在10 6M的维生素K2给药后1小时和6小时增加。在添加维生素D的情况下观察到相同的趋势。由于维生素K2可能影响细胞功能，因此采用抑制性消减杂交技术（SH）检测可能影响细胞功能的因素。在该方法中，我们检测到的基因，如tenascin，lumican等，需要进一步调查，以检测基因，这是受维生素K2。

项目成果

Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Effects of continuous intake of vitamin K_2 on bone turnover."Jpn. J. Hygiene. 56-1. 123-109 (2001)

Katsuyama, H.、Ideguchi, S.、Fukunaga, M.、Saijoh, K.、Sunami, S.：“连续摄入维生素 K_2 对骨转换的影响。”Jpn。

勝山博信: "マウス骨芽細胞に対するビタミンK_2投与の影響"日本衛生学雑誌. 57巻・1号. 378 (2002)

Hironobu Katsuyama：“维生素 K_2 给药对小鼠成骨细胞的影响”日本卫生杂志第 57 卷第 378 期（2002 年）。

Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Effects of vitamin K_2 intake and vitamin D receptor polymorphism on bone turnover."Jpn. J. Hygiene. 55-1. 449-162 (2000)

Katsuyama, H.、Ideguchi, S.、Fukunaga, M.、Saijoh, K.、Sunami, S.：“维生素 K_2 摄入量和维生素 D 受体多态性对骨转换的影响。”Jpn。

Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Influence of vitamin K_2 intake on bone turnover markers."J. Epidemiol. 12-1. 109-378 (2002)

Katsuyama, H.、Ideguchi, S.、Fukunaga, M.、Saijoh, K.、Sunami, S.：“维生素 K_2 摄入量对骨转换标志物的影响”。

Katsuyama, H., Ideguchi, S., Fukunaga, M., Saijoh, K., Sunami, S.: "Biochemical markers for bone turnover and vitamin K_2 intake."abstract for EXPERIMENTAL BIOLOGY '99 (at Washington, DC., USA ; 17-21 April 1999). -449

Katsuyama, H.、Ideguchi, S.、Fukunaga, M.、Saijoh, K.、Sunami, S.：“骨转换和维生素 K_2 摄入量的生化标记。”实验生物学 99 摘要（华盛顿特区，