Maintenance of bone mineral density in Senescence Accelerated MiceP6 (SAMP6)

衰老加速小鼠 P6 (SAMP6) 中骨矿物质密度的维持

• 批准号: 22500684
• 负责人: KATSUYAMA Hironobu
• 金额: $ 2.75万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500684/
• 关键词: 加齢; 老化; 老化促進マウス; ビタミンK2; 骨形態計測; 水浸拘束ストレス; 骨代謝; 骨代謝マーカー; 生理活性物質

The P6 strain of senescence-accelerated mice (SAMP6) exhibit features of skeletal aging including reduced bone mineral density (BMD), so that various physiological active agents affecting BMD could be examined. We developed the further bone resorption model using water-immersion restraint stress (WRS). Male SAMP6 were divided into three groups, i.e., control group, WRS (6 h/day, 5 times/wk) group, and WRS + vitamin K2 (VK2) (30 mg/kg s.c.) group. SAMP6 were suffered from this stress between 6 to 10 weeks old. BMD was measured using computed tomography (CT) system for small animals, and fluctuation of bone turnover markers and bone histomorphometrical analysis were also performed.BMD of trabecular bone for WRS groups was significantly lower than that of control group, suggesting that WRS was effective to reduce BMD. However, no significant difference of BMD was observed between WRS group and WRS + VK2 group. In bone turnover markers, urinary excretion of CTX, bone resorption mark，was significantly higher in WRS groups than in control. And urinary excretionof calcium was significantly higher in WRS group than in other groups. On the other hand, bone histomorphometrical analysis indicated that trabeculae of WRS showed sthin and mall shape, but that of WRS + VK2 showed larger and round shape. On the resorption area, large osteoclast was observed in WRS, but the number of osteoclasts was significantly decreased in WRS + VK2. These resultsindicated that vitamin K2 could affect bone turnover.

加速衰老小鼠P6品系（SAMP 6）表现出包括骨矿物质密度（BMD）降低在内的骨骼老化特征，因此可以检查影响BMD的各种生理活性剂。我们进一步发展了水浸束缚应力（WRS）骨吸收模型。将雄性SAMP 6分为三组，即，对照组、WRS（6 h/d，5次/wk）组和WRS +维生素K2（VK 2）（30 mg/kg s.c.）组SAMP 6在6 - 10周龄之间遭受这种应激。采用CT测量小动物骨密度，并进行骨转换指标的变化和骨组织形态计量学分析，结果表明，WRS组骨小梁骨密度明显低于对照组，表明WRS能有效降低骨密度。而WRS组与WRS + VK 2组的骨密度差异无统计学意义。在骨转换指标中，WRS组尿中骨吸收指标CTX排泄量显著高于对照组。尿钙排泄量WRS组明显高于其他各组。骨组织形态计量学分析显示，WRS组骨小梁细而小，而WRS + VK 2组骨小梁大而圆。在骨吸收区，WRS组可见大量破骨细胞，而WRS + VK 2组破骨细胞数量明显减少。提示维生素K2可影响骨转换。

项目成果

老化促進マウスを用いた骨密度減少モデルの作成

使用加速衰老的小鼠创建骨密度降低模型

• 发表时间: 2012
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信;勝山博信;勝山博信
• 通讯作者: 勝山博信

Vitamin K2 administration and bone mineral density in Senescence Accelerated Mice P-6 (SAMP6)

维生素 K2 给药与衰老加速小鼠 P-6 (SAMP6) 的骨矿物质密度

• 发表时间: 2012
• 作者: Hironobu Katsuyama;Shigeko Fushimi;Kunikazu Yamane;Hajime Hinenoya;Yuji Akiyama;Masafumi Tomita;Toshiko Okuyama;Yoko Watanabe;Midori Katsuyama;Le Ngoc Anh;Kiyofumi Saijoh
• 通讯作者: Kiyofumi Saijoh

マウス骨密度に及ぼす水浸拘束ストレスの影響

水浸约束应力对小鼠骨密度的影响

• 发表时间: 2012
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信;勝山博信
• 通讯作者: 勝山博信

ビタミンK2がストレスモデルマウスの骨形態におよぼす影響

维生素K2对应激模型小鼠骨形态的影响

• 发表时间: 2013
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信
• 通讯作者: 勝山博信

Water-restraint stress enhances methamphetamine-induced cardiotoxicity.

• DOI: 10.1016/j.cbi.2011.01.025
• 发表时间: 2011-03
• 期刊: Chemico-biological interactions
• 影响因子: 5.1
• 作者: M. Tomita;H. Katsuyama;Yoko Watanabe;Kazuo Hidaka;K. Yoshitome;S. Miyaishi;T. Ishikawa;Kotaro Shinone-Kota