Analysis of the gastric mucus as a host factor for the Helicobacter pylori infection.

胃粘液作为幽门螺杆菌感染宿主因素的分析。

• 批准号: 12670513
• 负责人: GOSO Yukinobu
• 金额: $ 2.05万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670513/
• 关键词: Helicobacter pylori; Monoclonal antibody; Mucus gel; Gastric mucus; Gastric mucosa; Oligosaccharides; Mucin; 粘膜ゲル層; ネオ糖脂質; MALDI-TOF-MS

In order to examine the role of the gastric mucus on Helicobacter pylori (Hp) infection, the following studies have been performed.1. Binding of human gastric mucins with Hp was assayed with the surface plasmon biosensors. The results indicated that Hp bound strongly to the gastric mucins obtained from the corpus compared to those from the antrum.2. A novel anti-mucin monoclonal antibody (mAb), designated RGM23, was developed and characterized. The histochemical and biochemical studies showed that RGM23 recognized the peptide moiety of MUC5AC mucin present in the surface mucosa of the stomach. Combined use of RGM23 and HIK1083, which recognize the mucin present in the glandular mucosa, may permit to get the surface and glandular mucins separately. This may enable to reveal a role of each mucin for Hp infection.3. To determine the mucin oligosaccharides recognized by Hp, binding assay methods were developed. Intact oligosaccharides were obtained from the mucins by gas-phase hydrazynolysis and then converted to neoglycolipid. After the neoglycolipids were separated by thin-layer chromatography, their mass were determined by MALDI-TOF-MS. Hp bind to oligosaccharides on TLC plates, thus enabling to determine structure of the oligosaccharides recognized by Hp.

为了研究胃粘液在幽门螺杆菌（Hp）感染中的作用，进行了以下研究.用表面等离子体生物传感器检测人胃粘蛋白与Hp的结合。结果表明，Hp与胃体粘蛋白的结合强于胃窦粘蛋白.本文报道了一种新的抗粘蛋白单克隆抗体RGM 23。组织化学和生物化学研究表明，RGM 23识别存在于胃表面粘膜中的MUC 5AC粘蛋白的肽部分。RGM 23和HIK 1083的组合使用，其识别存在于腺粘膜中的粘蛋白，可以允许分别获得表面粘蛋白和腺粘蛋白。这可能有助于揭示每种粘蛋白在Hp感染中的作用.为了确定被Hp识别的粘蛋白寡糖，开发了结合测定方法。通过气相肼解从粘蛋白中获得完整的寡糖，然后将其转化为新糖脂。新糖脂经薄层层析分离后，通过MALDI-TOF-MS测定其质量。Hp与TLC板上的低聚糖结合，从而能够确定Hp识别的低聚糖的结构。

项目成果

Y.Kojima, 他7名: "Ex-vivo Gastric Chamberによるラット胃粘膜の肉眼的観察法を用いたアスピリン起因性胃粘膜傷害に対するTeprenone前投与の検討"薬理と治療. 29. 245-251 (2001)

Y.Kojima等人7：“使用离体胃室对大鼠胃粘膜进行宏观观察来检查替普瑞酮对阿司匹林引起的胃粘膜损伤的影响”29.245-251(2001)。

Ikezawa T. et al.: "Immunohistochemical localization in rat gastrointestinal tract of a sialomucin species recognized by HCM31, a new anti-mucin monoclonal antibody."Biomed.Res.. 23. 63-68 (2002)

Ikezawa T.等人：“HCM31（一种新的抗粘蛋白单克隆抗体）识别的唾液酸粘蛋白在大鼠胃肠道中的免疫组织化学定位。”Biomed.Res.. 23. 63-68 (2002)

T.Ikezawa, 他5名: "Immunohistochemical localization in rat gastrointestinal tract of a sialomucin species recognized by HCM31, a new anti-mucin monoclonal antibody."Biomed.Res.. 23. 63-68 (2002)

T.Ikezawa 等 5 人：“HCM31（一种新的抗粘蛋白单克隆抗体）识别的唾液粘蛋白​​在大鼠胃肠道中的免疫组织化学定位。”Biomed.Res.. 23. 63-68 (2002)

Goso Y, et al.: "Characterization of rat gastric mucins using a monoclonal antibody, RGM23, recognizing surface mucous cell-type mucins."J.Biochem.. 133. 453-460 (2003)

Goso Y 等人：“使用单克隆抗体 RGM23 识别表面粘液细胞型粘蛋白来表征大鼠胃粘蛋白。”J.Biochem.. 133. 453-460 (2003)

Y.Goso 他4名: "Characterization of rat gastric mucins by anti-mucin monoclonal antibodies"Ulcer Res.. 28. 86-88 (2001)

Y. Goso 和其他 4 人：“抗粘蛋白单克隆抗体对大鼠胃粘蛋白的表征”Ulcer Res.. 28. 86-88 (2001)