THE EVOLUTION OF BLOOD COAGULATION FACTOR VIII IN MAMMALS

点击翻译按钮获取中文摘要

The coagulability in various mammals; monkeys, dogs, cats, pigs, cattle, mice and a killer whale were studied as compared to that in human. The levels of factor VIII (FVIII) coagulant activity in all mammals but monkeys were higher than that in human, resulting in their hypercoagulability in the intrinsic pathway of blood coagulation.Cross-reactivity of monoclonal antibodies against various mammalian FVIII was determined as compared to human FVIII. The cross-reactivity of C5 which recognized A1 domain of FVIII heavy chain were 7 % in monkeys, 2.7 % in dogs, 16.2 % in cats, and 0 % in the other mammals. The cross-reactivity of RFF/8, which recognized A2 domain of FVIII heavy chain were 1.3 % in monkeys, and 0 % in the others. No cross-reactivity of NMC-VIII/9, which recognized A3 domain of FVIII light chain, was observed in all mammals. The cross-reactivity of NMC-VIII/9, which recognized C2 domain of FVIII light chain were 54.5 % in monkeys, 130 % in dogs, 82.8 % in cats, 13.4 % in pigs, 0 % in the others. These data suggest that the stereoscopic structure of C2 epitope in the light chain of FVIII molecule is preserved among most of mammals and that C2 epitope is very important region to FVIII function.