Research and Development of Apoptosis Detection by PET.

PET检测细胞凋亡的研究进展。

• 批准号: 12670853
• 负责人: INOUE Tomio
• 金额: $ 1.34万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670853/
• 关键词: Apoptosis; PET; Annexin

Apoptotic death is a phenomenon in cellular unit, and the development of imaging the apoptotic death would be useful for resolving unknown pathophisiology of various disease, diagnoses, and therapeutic decision. The nuclear medicine imaging using radio nuclides might be the most sensitive and possible technology for imaging molecular biologic phenomenon such as apoptosis. In these technique, positron emission tomography (PET) has been focused because of its high quantitative imaging technology. This study was conducted in the PET facility in Gunma University. The aim of this study was to basically and clinically evaluate the relationship between the F-18 α methyltyrosine (FMT) as a amino-acid transporter marker, C-11 choline as a cellular membrane phospholipid metabolic marker and apoptotic phenomenon. Also we tried to develop new C-11 or F-18 labeled PET tracers detecting apoptotic phenomenon on in vivo images which were belonged to a phospholipids family. As a result, we could not find a significant correlation between FMT and C-11 choline tumor uptake and apoptotic phenomenon from clinical data analysis. We succeeded to generate a new tracer. C-11 Annexin, detecting apoptotic phenomenon. This new tracer showed a significant different cell biding ability between apoptosis free cells and apoptosis induced cells.C-11 Annexin appeared to be an arailable for detecting apoptotic cells and further investigation using in vivo animal study was warranted before clinical trials.

细胞凋亡性死亡是一种以细胞为单位的现象，细胞凋亡性死亡成像的发展将有助于解决各种疾病未知的发病机制、诊断和治疗决策。利用放射性核素进行核医学成像可能是对细胞凋亡等分子生物学现象进行成像的最敏感和最有可能的技术。在这些技术中，正电子发射断层成像(PET)因其高定量成像技术而备受关注。这项研究是在群马大学的PET设备中进行的。本研究的目的是从基础和临床上探讨作为氨基酸转运蛋白标志物的F-18α甲基酪氨酸和作为细胞膜磷脂代谢标志物的C-11胆碱与细胞凋亡现象的关系。此外，我们还试图开发新的C-11或F-18标记的PET示踪剂，在体内图像上检测属于磷脂家族的细胞凋亡现象。因此，从临床资料分析中，我们没有发现FMT与C-11胆碱摄取和肿瘤细胞凋亡现象之间存在显著的相关性。我们成功地产生了一种新的示踪剂。C-11 Annexin，检测细胞凋亡现象。这种新的示踪剂在无凋亡细胞和诱导凋亡细胞之间显示了显著不同的结合能力。C-11 Annexin似乎是一种检测凋亡细胞的可行方法，在临床试验之前，还需要进行进一步的动物体内研究。

项目成果

Toshiyuki Ohtani: "Brain tumour imaging with carbon-11 choline:comparison with FDG PET and gadolinuium-enhanced MR imaging"Eur J Nucl Med. 28. 1664-1670 (2001)

Toshiyuki Ohtani：“碳 11 胆碱脑肿瘤成像：与 FDG PET 和钆增强 MR 成像的比较”Eur J Nucl Med。

Yoshiki Nakasone.: "The role of whole-body FDG-PET in preoperative assessment of tumor staging in oral cancers"Ann Nucl Med. 15. 505-512 (2001)

Yoshiki Nakasone.：“全身 FDG-PET 在口腔癌肿瘤分期术前评估中的作用”Ann Nucl Med。

Inoue Tomio: "Preliminary studyof whole-body PET using F-18 alpha-methyl tyrosine in the detection of malignant tumors : Comparison with whole-body FDG PET"Radiology. (in press). (2001)

Inoue Tomio：“使用 F-18 α-甲基酪氨酸检测恶性肿瘤的全身 PET 的初步研究：与全身 FDG PET 的比较”放射学。

Tomio Inoue: "Detection of malignant tumors : Whole-body PET with fluorine 18 α-methyl tyrosine versus FDG preliminary study"Radiology. 220. 54-62 (2001)

Tomio Inoue：“恶性肿瘤的检测：氟 18 α-甲基酪氨酸与 FDG 的全身 PET 对比初步研究”放射学。 220. 54-62 (2001)

井上登美夫: "FDG-PETによる肺癌の診断"肺癌の臨床. 4. 157-162 (2001)

Tomio Inoue：“FDG-PET 诊断肺癌”《临床肺癌》，4. 157-162 (2001)。