Research and Development of Apoptosis Detection by PET.

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Apoptotic death is a phenomenon in cellular unit, and the development of imaging the apoptotic death would be useful for resolving unknown pathophisiology of various disease, diagnoses, and therapeutic decision. The nuclear medicine imaging using radio nuclides might be the most sensitive and possible technology for imaging molecular biologic phenomenon such as apoptosis. In these technique, positron emission tomography (PET) has been focused because of its high quantitative imaging technology. This study was conducted in the PET facility in Gunma University. The aim of this study was to basically and clinically evaluate the relationship between the F-18 α methyltyrosine (FMT) as a amino-acid transporter marker, C-11 choline as a cellular membrane phospholipid metabolic marker and apoptotic phenomenon. Also we tried to develop new C-11 or F-18 labeled PET tracers detecting apoptotic phenomenon on in vivo images which were belonged to a phospholipids family. As a result, we could not find a significant correlation between FMT and C-11 choline tumor uptake and apoptotic phenomenon from clinical data analysis. We succeeded to generate a new tracer. C-11 Annexin, detecting apoptotic phenomenon. This new tracer showed a significant different cell biding ability between apoptosis free cells and apoptosis induced cells.C-11 Annexin appeared to be an arailable for detecting apoptotic cells and further investigation using in vivo animal study was warranted before clinical trials.