The Effect of L-Valine on the Intestinal Mucosal Regeneration

L-缬氨酸对肠粘膜再生的影响

• 批准号: 12671193
• 负责人: DOI Hideyuki
• 金额: $ 2.18万
• 依托单位: TOHOKU University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671193/
• 关键词: Valine; Glutamine; 5-fluorouracil; small intestinal mucosa; BCAAs; antagonism; regeneration; 誘発電位; Autoradiography; 吸収; BCAA; ^<14>C-Val; 分岐鎖アミノ酸; L-Valine; Glutamine; 5-fluorouracil(5-Fu); 再生

Purpose : This study was conducted to determine the role of individual branched-chain amino acids (BCAAs) and antagonism on the growth and differentiation of the small intestinal mucosa. Method : Male Donryu rats were used for all experiments. Immediately after the catheterization, each group received different infusion solutions as described below, and all rats were injected 5-fluorouracil (5-FU) intraperitoneally at 40 mg/kg/day. Control group (C group) received continuous infusions of TPN containing balanced 10 % amino acids preparation for 6 days. Val group received the TPN solution added with Val. Glutamine group (G group) received glutamine enriched TPN. Leu group, Ile group, Val+Leu(VL) group and Val+Ile(VI) group received the TPN solutions fortified with BCAAs. On the day 6, villous height, surface area, mucosal wet weight and protein were analyzed. Results : Villous height and surface area of jejunum was significantly higher in V group and G group compared with that in C group. The mean villous height of jejunum was significantly higher in V and VI group than those in C, Leu, Ile and VL group. Surface area of jejunum was significantly wider in V and VI group than those in C, Leu and Ile group. Villous height and surface area of ileum was significantly higher in V and VI group than those in C, Leu and Ile group. Conclusions : Only Val among BCAAs was first found to be effective in the recovery of injury of the small intestinal mucosa. But the effect of Val was inhibited by Leucine.

目的：本研究旨在探讨支链氨基酸（BCAAs）对小肠粘膜生长和分化的作用及其拮抗作用。方法：所有实验均使用雄性Donryu大鼠。导管插入后，各组立即接受下述不同的输注溶液，所有大鼠均以40 mg/kg/天的剂量腹腔内注射5-氟尿嘧啶（5-FU）。对照组（C组）连续输注含10%平衡氨基酸制剂的TPN 6天.瓦尔组给予添加瓦尔的TPN溶液。谷氨酰胺组（G组）给予谷氨酰胺强化TPN。Leu组、Ile组、瓦尔+Leu（VL）组和瓦尔+Ile（VI）组给予BCAA强化的TPN。在第6天，分析绒毛高度、表面积、粘膜湿重和蛋白质。结果：V组和G组空肠绒毛高度和表面积均显著高于C组。空肠平均绒毛高度V、VI组显著高于C、Leu、Ile和VL组。V、VI组空肠表面积较C、Leu、Ile组明显增大。回肠绒毛高度和表面积V、VI组显著高于C、Leu和Ile组。结论：在支链氨基酸中，只有瓦尔对小肠粘膜损伤的修复有较好的效果。但瓦尔的作用可被Leucine抑制。