Protein-protein interactions in mono- and bi-partite geminiviruses: functional analysis and use of aptamers for plant protection

单分体和双分体双生病毒中蛋白质-蛋白质相互作用：功能分析和适体在植物保护中的应用

基本信息

批准号：
53100863
负责人：
Professor Dr. Holger Jeske (†)
金额：
--
依托单位：
Forschungseinheit Molekulare und Synthetische Pflanzenvirologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2007
资助国家：
德国
起止时间：
2006-12-31 至 2013-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/53100863?language=en
关键词：
Protein protein interactions mono bi

项目摘要

This proposal represents a continuation of our ongoing efforts to study, identify and characterize the molecular components and the processes involved in infection, spread and pathogenicity of plant ssDNA viruses from the Geminiviridae family: Tomato yellow leaf curl virus (TYLCV), Watermelone chlorotic stunt virus (WmCSV), and Indian cassava mosaic virus (ICMV). The information gained will be used to develop novel strategies to render plants resistant to geminivirus infection. This will be achieved by expressing inhibitory peptides in planta that will interfere with inter- and intracellular movement of geminiviral proteins. A random peptide library and the yeast two hybrid (Hoppe-Seyler et al., 2004) as well as the reverse two-hybrid (Barr et al., 2004) systems will be used to select such inhibitory peptides. Indeed, we have already succeeded, using Y2H system in selecting a peptide that inhibits virus replication (HIV-l) in cultured cells (Armon-Omer et al., 2007). The same strategy will be used by us in the present project Engineering of virus-resistant transgenic plants by expressing dominant interfering peptides (aptamers) was described recently as a novel and feasible approach in order to control an RNA virus infection (Rudolph et a/., 2003) as well as the replication of a geminivirus (Lopez-Ochoa et aly 2006). Here, we propose to exploit our knowledge on the use of random peptide libraries and on the interactions of geminivirus proteins to generate inhibitory peptides to obtain virus resistant plants.

This proposal represents a continuation of our ongoing efforts to study, identify and characterize the molecular components and the processes involved in infection, spread and pathogenicity of plant ssDNA viruses from the Geminiviridae family: Tomato yellow leaf curl virus (TYLCV), Watermelone chlorotic stunt virus (WmCSV), and Indian cassava mosaic virus (ICMV).获得的信息将用于制定新型策略，以使植物具有抗双子病毒感染的植物。这将通过在植物中表达抑制性肽来实现，这将干扰双子病毒蛋白的细胞内和细胞内运动。随机肽库和酵母两个混合动力（Hoppe-Seyler等，2004）以及反向两杂交（Barr等，2004）系统将用于选择这种抑制性肽。确实，我们已经成功地使用Y2H系统选择了抑制培养细胞中病毒复制（HIV-L）的肽（Armon-Omer等，2007）。 The same strategy will be used by us in the present project Engineering of virus-resistant transgenic plants by expressing dominant interfering peptides (aptamers) was described recently as a novel and feasible approach in order to control an RNA virus infection (Rudolph et a/., 2003) as well as the replication of a geminivirus (Lopez-Ochoa et aly 2006).在这里，我们建议利用我们对使用随机肽文库的使用以及双子蛋白的相互作用来产生抑制性肽以获得抗病毒植物。