BMP Signaling on Cartilage Repair Response in Full-thickness Articular Cartilage Defects

我们研究了BMP信号对全层关节软骨缺损中软骨修复反应的影响。在先前的研究中，我们建立了一个大鼠模型，以阐明软骨修复反应的全层关节软骨缺损。分别于术后第2、4、7、14天采用免疫组织化学染色和原位杂交方法观察大鼠小缺损（0.7mm宽）和大缺损（1.5mm宽）的修复情况。在术后2天，在小缺损的中心发现了BMP-4的信使RNA，但未观察到这种蛋白质。此后，发现BMP-4的信使RNA和蛋白贯穿各个时期。BMP-6的mRNA和蛋白在术后第7天首次被发现。GDF-5的mRNA和蛋白在术后第4天开始出现。这些受体的信使RNA和蛋白质在所有时期都有表达。另一方面，在大缺损的中心，在手术后2天，BMP-4的信使RNA被发现，没有蛋白质。术后4d，BMP-4 mRNA和蛋白质有少量表达。术后第7天，仅观察到BMP-4的信使RNA。术后7 d未检测到BMP-4的mRNA和蛋白表达，BMP-6和GDF-5的mRNA和蛋白表达在各时间段均未检测到。这些受体的信使RNA和蛋白质在所有时期都被发现，以及那些在一个小的缺陷。基于本研究的结果，证实了在全层关节软骨缺损的软骨修复过程中，BMP-4信号对于该过程的启动是重要的，BMP-6信号可能参与软骨细胞的成熟和肥大，GDF-5信号可能参与软骨细胞的肥大

We investigated BMP signaling on cartilage repair response in full-thickness articular cartilage defects. In the previous study, we established a rat model to clarify cartilage repair response full-thickness articular cartilage defects. The repair process in the center of the defects was analyzed by immunostaining and in situ hybridyzation method at 2,4,7,14 days after surgery using our rat model with a small defect (0.7mm width) and a large defect (1.5mm width). In the center of a small defect, at 2 days after surgery, the messenger RNA of BMP-4 was found, but this protein was not observed. After that, the messenger RNA and protein of BMP-4 was found through all periods. The messenger RNA and protein of BMP-6 was first found at 7 days after surgery. The messenger RNA and protein of GDF-5 was slightly first found at 4 days after surgery. The messenger RNA and protein of those receptors was expressed through all periods. On the other hand, in the center of a large defect, at 2 days after surgery, the messenger RNA of BMP-4 was found without no protein. At 4days after surgery, the messenger RNA and protein of BMP-4 was slightly expressed. At 7 days after surgery, the messenger RNA of BMP-4 was only observed. At 7 days after surgery, the messenger RNA and protein of BMP-4 were not found. The messenger RNA and protein of BMP-6 and GDF-5 was not observed through all periods. The messenger RNA and protein of those receptors was found through all periods as well as those in a small defects. Based on the results of this study, it was certified that, on cartilaginous repair in full-thickness articular cartilage defects, BMP-4 signaling is important for the initiation of the process, BMP-6 signaling may be involved in chondrocyte maturation and hypertrophy, GDF-5 signaling may be involved in chondrocyte hypertrophy