Differentiation of potential of umbilical cord blood cells into cartilage and ligament cells.

脐带血细胞分化为软骨和韧带细胞的潜力。

• 批准号: 12671433
• 负责人: TAKAI Shinro
• 金额: $ 2.24万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671433/
• 关键词: umbilical cord blood; transgenic rat; ligament; transplantation; mesenchymal cells; osteochondral defect; Hangking Drop Culture; in situ hybridization; in situ hybridization; 骨髄細胞; Hanging Drop法; 細胞培養; in situ hybridaization法

In order to elucidate the differentiation potential of umbilical cord blood cells into chondrocytes and ligament cells, first of all, transplantation of bone marrow-derived mesenchymal cells of rats were applied to osteochondral defect and medial collateral ligament (MCL) injury of rats. The autologous transplantation model using transgenic rat was established, which cells were able to detect by in situ hybridization. The hanging drop culture method was first applied to mesenchymal cells and the cultured mesenchymal cells were transplanted into osteochondral defect.Transplanted cells survived at MCL injury site for 4 weeks. These results will be published in Microscopy Research Technique (MRT) (in press). Furthermore, osteochondral defect was filled with hyaline cartilage-like tissue 12 weeks after transplantation of bone marrow mesenchymal cells, which were cultivated by hanging drop culture. The survival of transplanted cells was also found. The differentiation potential of bone marrow-derived mesenchymal cells into chondrocytes was thus demonstrated in vivo. These results were presented at 106^<th> Japanese Association of Anatomists and 48th orthopaedic research society (Dallas, USA).For understanding the fate of host cells as well as donor cells, the transplantation model, in which osteochondral fragment was transplanted reversely in transgenic rat and its wild-type rat, was already completed. These results were presented at 42nd Japan society of histochemistry and cytochemistry and will be published in MRT (in press).

为了探讨脐血细胞向软骨细胞和韧带细胞的分化潜能，首先将大鼠骨髓间充质细胞移植于骨软骨缺损和内侧副韧带（MCL）损伤的大鼠。建立了转基因大鼠自体移植模型，原位杂交检测到细胞。采用悬滴法培养骨髓间充质细胞，将培养的骨髓间充质细胞移植于骨软骨缺损处，移植细胞在MCL损伤处存活4周。这些结果将发表在《显微镜研究技术》（MRT）上（出版中）。悬滴法培养的骨髓间充质细胞移植12周后，骨软骨缺损处可见透明软骨样组织填充。此外，还发现了移植细胞的存活，从而在体内证明了骨髓间充质细胞向软骨细胞的分化潜力。这些结果在106届<th>日本解剖学会和第48届矫形外科研究会（达拉斯，美国）上发表。为了了解宿主细胞和供体细胞的命运，已经完成了在转基因大鼠及其野生型大鼠中同时移植骨软骨碎片的移植模型。这些结果在第42届日本组织化学和细胞化学学会上发表，并将在MRT上发表（出版中）。

项目成果

Yasushi Oshima, et al.: "Behavior of Graft and Host Cells in Underlying Subchondral Bone after Transplantation of Osteochondral Autograft"Microscopic Research Technique. 58:1(in press). (2002)

Yasushi Oshima 等人：“自体骨软骨移植后软骨下骨中移植物和宿主细胞的行为”显微研究技术。

Yasushi Oshima, et al.: "A Novel Method to Follow the Regenration Process of Osteochondral defect by Transplantation of Mesenchymal Cell Masses"Transaction of Orthopaedic Research Society. 27. 253 (2002)

Yasushi Oshima 等人：“通过间充质细胞团移植追踪骨软骨缺损再生过程的新方法”骨科研究会汇刊。

Nobuyoshi Watanabe, et al.: "Introduction for "Donor transplant vs. recipient cells in musculoskeletal system""Microscopic Research Technique. 58:1(in press). (2002)

Nobuyoshi Watanabe 等人：“肌肉骨骼系统中供体移植与受体细胞的简介”显微研究技术。

Nobuyoshi Watanabe et al.: "Introduction for "Donor transplant vs. recipient cells in musculoskeletal system""Microscopic Reserach Technique. 58 : 1 (in press). (2002)

Nobuyoshi Watanabe 等人：“肌肉骨骼系统中“供体移植与受体细胞”简介“显微研究技术”。

Nobuyoshi Watanabe, et al.: "Introduction for "Donor transplant vs.recipient cells in musculoskeletal system""Microscopic Resarch Technique. 58:1(in press). (2002)

Nobuyoshi Watanabe 等人：“肌肉骨骼系统中“供体移植与受体细胞”简介“显微研究技术。