Differentiation of potential of umbilical cord blood cells into cartilage and ligament cells.

为了阐明脐带血细胞向软骨细胞和韧带细胞分化的潜能，首先将大鼠骨髓间充质细胞移植到大鼠骨软骨缺损和内侧副韧带（MCL）损伤中。建立了转基因大鼠的自体移植模型，并进行原位杂交检测。首次应用悬滴培养法培养间充质细胞，将培养的间充质细胞移植到骨软骨缺损中。移植细胞在MCL损伤部位存活4周。这些结果将发表在《显微研究技术》（MRT）杂志上。移植骨髓间充质细胞12周后，骨软骨缺损内充满透明软骨样组织，采用悬挂滴培养法培养。移植细胞也能存活。骨髓间充质细胞向软骨细胞的分化潜力在体内得到了证实。这些研究结果发表于2010年日本解剖学家协会和第48届骨科研究学会（Dallas， USA）。为了了解宿主细胞和供体细胞的命运，已经完成了将骨软骨片段反向移植到转基因大鼠及其野生型大鼠身上的移植模型。这些结果在第42届日本组织化学和细胞化学学会上发表，并将在MRT上发表。

In order to elucidate the differentiation potential of umbilical cord blood cells into chondrocytes and ligament cells, first of all, transplantation of bone marrow-derived mesenchymal cells of rats were applied to osteochondral defect and medial collateral ligament (MCL) injury of rats. The autologous transplantation model using transgenic rat was established, which cells were able to detect by in situ hybridization. The hanging drop culture method was first applied to mesenchymal cells and the cultured mesenchymal cells were transplanted into osteochondral defect.Transplanted cells survived at MCL injury site for 4 weeks. These results will be published in Microscopy Research Technique (MRT) (in press). Furthermore, osteochondral defect was filled with hyaline cartilage-like tissue 12 weeks after transplantation of bone marrow mesenchymal cells, which were cultivated by hanging drop culture. The survival of transplanted cells was also found. The differentiation potential of bone marrow-derived mesenchymal cells into chondrocytes was thus demonstrated in vivo. These results were presented at 106^<th> Japanese Association of Anatomists and 48th orthopaedic research society (Dallas, USA).For understanding the fate of host cells as well as donor cells, the transplantation model, in which osteochondral fragment was transplanted reversely in transgenic rat and its wild-type rat, was already completed. These results were presented at 42nd Japan society of histochemistry and cytochemistry and will be published in MRT (in press).