The Origin of the repaired cells and function of mesenchymal stem cell

本研究对修复细胞的来源和间充质干细胞在组织修复中的作用进行了研究。为了在体内跟踪移植细胞，建立了消除宿主对供体细胞免疫排斥反应的移植模型。首先，将各种类型的细胞移植到组织中，以证明该模型在跟踪移植细胞方面的完整性。然后，利用该移植模型对骨髓间充质干细胞的分化进行了研究。本研究以雌性转基因大鼠为供体，以雌性野生型大鼠为受体。由于这些大鼠之间的DNA在理论上是一致的，除了转基因基因外，移植细胞可以通过与转基因大鼠细胞的转基因基因的原位杂交在受体组织中进行追踪。此外，由于使用的是原本在全身都含有转基因的转基因大鼠，因此不需要引入标记。使用…此外，在这一新的移植模型中，通过对BrdU的免疫组织化学染色，发现移植的滑膜细胞在关节内存活和增殖至少4周(Trans ORS 1998，Cell Tucture Res 1999)。通过结合新开发的微型压痕器，还发现移植的骨软骨碎片中的供体细胞导致了软骨的个体发育，并有助于粘弹性的恢复(Trans ORS 1999)。因此，这种移植模型有利于利用生物和生物力学方法对组织修复进行多学科研究。另一方面，众所周知，骨髓是间充质干细胞的来源之一。在本研究中，将转基因大鼠的骨髓细胞移植到野生型大鼠膝关节损伤的内侧副韧带中。对这些细胞的分化进行了形态观察。结果，骨髓细胞被证明可以分化为成纤维细胞。这些细胞也被发现迁移到中间物质中，并协助构成愈合的MCL(Hilton Head Shop，1999)。较少

In this study the origin of the repaired cells and the function of mesenchymal stem cells in tissue healing were examined. The transplantation model to eliminate the immunological rejection of the donor cells by the host was developed in order to follow transplanted cells in vivo. First, various kinds of cells were transplanted into tissues to prove the integrity of this model in following the transplanted cells. Then, the differentiation of mesenchymal stem cells was studied using this transplantation model. In this study, a female transgenic rat was used as a donor, and a female wild-type rat was used as a recipient. Since the DNA between these rats is theoretically coincident except for transgenes, transplanted cells could be followed in the recipient tissues by in situ hybridization against transgenes of transgenic rats cells. Furthermore, due to the use of the transgenic rat, which originally contains transgenes in the whole body, the introduction of markers is not necessary. Usin … More g this novel transplantation model with immunohistochemistry against BrdU, it was found that transplanted synovial cells survive and proliferate in the joint for at least 4 weeks (Trans ORS 1998, Cell Tissue RES 1999). By combining the newly developed micro-indentator, it was also found that donor cells in the transplanted osteochondral fragment caused the enchondral ontogenesis and helped in the recovery of viscoelastic properties (Trans ORS 1999). This transplantation model therefore was beneficial in performing multidisciplinary study on the repair of the tissue, using biological and biomechanical approaches. On the other hand, bone marrow is well known as a source of mesenchymal stem cells. In this study, bone marrow cells of the transgenic rat were transplanted into the injured medial collateral ligament (MCL) of the knee in the wild-type rat. The differentiation of these cells was morphologically examined. As a result, bone marrow cells were demonstrated to differentiate into fibroblasts. These cells were also found to migrate into the midsubstance and assisted in constitute the healing MCL (Hilton head workshop 1999). Less