Regeneration of articular cartilage by high density bone marrow mesenchymal cells and bone morphological protein

高密度骨髓间充质细胞和骨形态蛋白促进关节软骨再生

• 批准号: 17591599
• 负责人: TAKAI Shinro
• 金额: $ 2.24万
• 依托单位: Teikyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591599/
• 关键词: articular cartilage; osteochondral defect; tissue regeneration; tidemark; mesenchymal cell; autologous cell transplantation; transgenic rat; in situ hybridization

The research which used the autologous cell graft for joint cartilage defect restoration has accomplished mostly. Restoration of normal hyaline cartilage has not been attained. We have completed the method for distinguishing donor and recipient cells using the combination of transgenic rat and DNA in situ hybridization. We reported those in the 48th and 49th Annual Meeting of the Orthopaedic Research Society (ORS, US), it was clearly demonstrated that cells from surrounding bone marrow replace the underlining bone of the donor with the donor cells. The model of following the transplanted cells was completed using GFP transgenic rats. This method is more reliable than the previously reported method using transgenic rat and DNA in situ hybridization. Transplantation of osteochondral autograft is widely used as a therapeutic strategy for the full thickness defect of articular cartilage. Although the viable graft has been demonstrated after the transplantation, the fate of host (intrinsic) … More and donor (extrinsic) cells and their effect on the biomechanical properties have not been elucidated. The objective of this study was to follow intrinsic and extrinsic cells and to examine the biomechanical properties in the healing tissue after transplantation of osteochondral autograft, An autologous transplantation model was used, in which a donor transgenic rat and recipient wild-type rat was genetically identical each other except for transgenes. It was hypothesized that intrinsic and extrinsic cells contribute to the regeneration of osteochondral defect after the transplantation of osteochondral autograft and affect viscoelastic properties of healing autograft. Intrinsic and extrinsic cells were clearly detected up to 12 weeks after transplantation. Extrinsic cells began to be identified close to the newly synthesized bone in the thickened cartilage at 6 weeks, and the regeneration of the subchondral bone completed by 12 weeks. It was also demonstrated that the remodeling of the subchondral bone occurred by enchondral ossification of extrinsic chondrocytes. Viscoelastic properties of the autograft at 6 weeks became inferior compared to those of the control, followed by the recovery up to control level by 12 weeks while the enchondral ossification was found. Less

应用自体细胞移植修复关节软骨缺损的研究已基本完成。尚未恢复正常的透明软骨。我们建立了转基因大鼠与DNA原位杂交相结合的供、受体细胞鉴别方法。我们在第48届和第49届骨科研究学会年会（ORS，US）上报道了这一结果，明确表明来自周围骨髓的细胞用供体细胞替代供体的底层骨。使用GFP转基因大鼠完成移植细胞的跟踪模型。该方法比以往报道的转基因大鼠法和DNA原位杂交法更可靠。自体骨软骨移植是治疗关节软骨全层缺损的常用方法。虽然移植后移植物已被证明是有活力的，但宿主的命运（内在的） ...更多信息 和供体（外源性）细胞以及它们对生物力学性质的影响尚未阐明。本研究的目的是跟踪内源性和外源性细胞，并检查骨软骨自体移植物移植后愈合组织的生物力学特性。使用自体移植模型，其中供体转基因大鼠和受体野生型大鼠除了转基因之外彼此遗传上相同。认为自体骨软骨移植后骨软骨缺损的再生可能与内源性和外源性细胞有关，并可能影响愈合的自体骨软骨的粘弹性。移植后12周内，可清楚地检测到内源性和外源性细胞。6周时，在增厚的软骨中，在新合成的骨附近开始发现外源性细胞，12周时软骨下骨的再生完成。研究还表明，软骨下骨的重塑是通过外源性软骨细胞的内骨化发生的。与对照组相比，6周时自体移植物的粘弹性变差，随后在12周时恢复到对照组水平，同时发现软骨内骨化。