Regeneration of articular cartilage by high density bone marrow mesenchymal cells and bone morphological protein

利用自体细胞移植修复关节软骨缺损的研究已基本完成。未达到正常透明软骨的修复。我们完成了利用转基因大鼠和DNA原位杂交相结合的方法来区分供受者细胞。我们在美国骨科学会第48届和第49届年会上报道了这一结果，明确表明来自周围骨髓的细胞用供体细胞取代供体的基础骨。用GFP转基因大鼠建立移植细胞跟踪模型。该方法比以往报道的转基因大鼠和DNA原位杂交方法更可靠。自体骨软骨移植被广泛应用于关节软骨全层缺损的治疗。尽管移植后证实了移植物的存活，宿主(固有的)…的命运More和供体(外源性)细胞及其对生物力学特性的影响尚未阐明。本研究采用自体移植模型，供体转基因大鼠与受体野生型大鼠在基因上完全相同，但转基因大鼠与受体野生型大鼠在基因上完全相同，观察自体骨软骨移植后愈合组织的生物力学特性。推测内、外源性细胞参与了自体骨软骨移植后骨软骨缺损的再生，并影响自体骨软骨移植愈合后的粘弹性。移植后12周，内源性和外源性细胞均清晰可见。6周时增厚的软骨内新合成的骨附近开始有外源性细胞出现，12周时软骨下骨的再生完成。研究还表明，软骨下骨的改建是由外源性软骨细胞的软骨化引起的。自体移植骨的粘弹性在6周时低于对照组，12周时恢复到对照组水平，但有软骨骨化。较少

The research which used the autologous cell graft for joint cartilage defect restoration has accomplished mostly. Restoration of normal hyaline cartilage has not been attained. We have completed the method for distinguishing donor and recipient cells using the combination of transgenic rat and DNA in situ hybridization. We reported those in the 48th and 49th Annual Meeting of the Orthopaedic Research Society (ORS, US), it was clearly demonstrated that cells from surrounding bone marrow replace the underlining bone of the donor with the donor cells. The model of following the transplanted cells was completed using GFP transgenic rats. This method is more reliable than the previously reported method using transgenic rat and DNA in situ hybridization. Transplantation of osteochondral autograft is widely used as a therapeutic strategy for the full thickness defect of articular cartilage. Although the viable graft has been demonstrated after the transplantation, the fate of host (intrinsic) … More and donor (extrinsic) cells and their effect on the biomechanical properties have not been elucidated. The objective of this study was to follow intrinsic and extrinsic cells and to examine the biomechanical properties in the healing tissue after transplantation of osteochondral autograft, An autologous transplantation model was used, in which a donor transgenic rat and recipient wild-type rat was genetically identical each other except for transgenes. It was hypothesized that intrinsic and extrinsic cells contribute to the regeneration of osteochondral defect after the transplantation of osteochondral autograft and affect viscoelastic properties of healing autograft. Intrinsic and extrinsic cells were clearly detected up to 12 weeks after transplantation. Extrinsic cells began to be identified close to the newly synthesized bone in the thickened cartilage at 6 weeks, and the regeneration of the subchondral bone completed by 12 weeks. It was also demonstrated that the remodeling of the subchondral bone occurred by enchondral ossification of extrinsic chondrocytes. Viscoelastic properties of the autograft at 6 weeks became inferior compared to those of the control, followed by the recovery up to control level by 12 weeks while the enchondral ossification was found. Less