Analysis of integrins in lymphnode metastasis of bladder cancer

膀胱癌淋巴结转移中整合素的分析

• 批准号: 12671560
• 负责人: KIYOTA Hiroshi
• 金额: $ 2.11万
• 依托单位: Jikei University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671560/
• 关键词: bladder cancer; lymphnode metastasis; integrin

We compared integrins expression of bladder cancers to those of their lymphnode metastasis in animal model, in order to clarify the role of integrins in the lymphnode metastasis of bladder cancer. The animal model of lymphnode metastsis was created by means of foot pad implantation method using human bladder cell lines, UM-UC-3 and UM-UC-13, and each integrin subunit was detected immuno-tissue stain (tissue ABC method and tissue fluorescent method) using monoclonal antibody against each integrin subunit. We found no difference in integrin expression between primary foot pad lesion and lymphnode metastasis. In addition, highly metastatic cells was not created from UM-UC-3 and UM-UC-13. We also tried to create highly metastatic cells from bladder implantation using UM-UC-3 and UM-UC-13, however, no lymphnode metastasis was found from bladder implantation. In conclusion, other tumor cell lines should be used in the same experimental model, or clinical speciemens should be investigated, in the future.

本研究通过比较膀胱癌及其淋巴结转移动物模型中整合素的表达，探讨整合素在膀胱癌淋巴结转移中的作用。采用人膀胱癌细胞系UM-UC-3和UM-UC-13，通过足垫植入法建立淋巴结肿大动物模型，并采用针对各整合素亚基的单克隆抗体进行免疫组织染色（组织ABC法和组织荧光法）检测各整合素亚基。我们发现整合素在原发性足垫病变和淋巴结转移之间的表达没有差异。此外，从UM-UC-3和UM-UC-13没有产生高转移性细胞。我们还尝试使用UM-UC-3和UM-UC-13从膀胱植入中产生高转移性细胞，然而，从膀胱植入中未发现淋巴结转移。因此，今后应在同一实验模型中使用其他肿瘤细胞系，或研究临床标本。