Development for model system of the relationship between fertility and oxydative on Ovary

生育力与卵巢氧化作用关系模型系统的研制

• 批准号: 12671611
• 负责人: YOSHIMURA Yasuhide
• 金额: $ 2.05万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671611/
• 关键词: Ovary; mutation; MTH1; MSH2; Knockout mouse; radiation; rpsL; EGFP

We have found the increase of mutation frequency on somatic cells by the analysis for MTH1 gene knockout mouse (KO mouse) by using rpsL gene system. We also found the problem due to the repeat sequences of rpsL gene in this system. The repeat sequences cause sequence slippage at the replication and that directly influence to the mutation frequency. For improve this problem, we have constructed the non-run rpsL gene system and TG mouse by using non-run rpsL gene. We also develop another system using GFP. We introduce the mutation on the GFP and by checking the number of mutated cells that had the reverse mutation.On the other hand, we checked the effect of radiation for MTH1 and MSH2 KO mouse. In MSH2 KO mouse, we recognize the apparent reduction of fertility.

我们用rpsL基因系统对MTH1基因敲除小鼠（KO小鼠）进行分析，发现体细胞突变频率增加。我们还发现了rpsL基因的重复序列在该系统中存在的问题。重复序列在复制过程中引起序列滑移，直接影响突变频率。为了解决这一问题，我们构建了非运行型rpsL基因系统，并利用非运行型rpsL基因构建了TG小鼠。我们还开发了另一个系统使用GFP。我们在GFP上引入突变，并通过检测具有回复突变的突变细胞的数量，另一方面，我们检测了辐射对MTH1和MSH2 KO小鼠的影响。在MSH2 KO小鼠中，我们发现生育力明显降低。

项目成果

Bain G et al.: "ES cell neural differenciation reveals a substantial number of novel ESTs"Funct Inter Genomics. 1. 127-139 (2000)

Bain G 等人：“ES 细胞神经分化揭示了大量新的 EST”Funct Inter Genomics。

Y.Yoshimura et al.: "Nested genomic structure of haploid germ cell specific haspin gene"Gene. 1(accepted on Feb.9th). (2001)

Y.Yoshimura等人：“单倍体生殖细胞特异性haspin基因的嵌套基因组结构”基因。

Tsuzuki T et al.: "Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase"Proc Natl Acad Sci USA.. Sep 25;98(20). 11456-11461 (2001)

Tsuzuki T 等人：“编码 8-oxo-dGTPase 的 MTH1 基因缺陷的小鼠的自发性肿瘤发生”Proc Natl Acad Sci USA.. Sep 25;98(20)。

Liang R a et al.: "Presence of potential nickel-responsive element(s) in the mouse MTH1 promoter"Ann Clin Lab Sci.. Jan;31(1). 91-98 (2001)

Liang R a 等人：“小鼠 MTH1 启动子中存在潜在的镍反应元件”Ann Clin Lab Sci.. Jan；31(1)。

Liang R aet al.: "Presence of potential nickel-responsive element(s) in the mouse MTH1 promoter"Ann Clin Lab Sci.. Jan;31(1). 91-98 (2001)

Liang R aet al.：“小鼠 MTH1 启动子中存在潜在的镍反应元件”Ann Clin Lab Sci.. Jan；31(1)。