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Recovery of vestibular function by intracochlear administration of various kinds of agents

通过耳蜗内给药各种药物恢复前庭功能

基本信息

批准号：
12671669
负责人：
SHIMOGORI Hiroaki
金额：
$ 1.54万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671669/
关键词：
steroid ATP vestibular disorder post-rotatory nystagmus osmotic pump vestibulo-ocular reflex 前庭眼反射利得

项目摘要

1 Drug effects on vestibular disorderEffects of steroid and ATP on peripheral vestibular system in guinea pig with vestibular disorder were investigated. The right lateral semicircular canal was surgically transected and lymph fluids were suctioned for two seconds. The transected ends were sealed with fibrin glue. After cochleotomy, betamethasone or ATP was directly infused into scala tympany with an osmotic pump. Before and after treatment, trapezoid rotation tests were performed and post-rotatory nystagmus was recorded on videotape with CCD camera in the dark, and was analyzed. Both betamethasone and ATP significantly improved post-rotatory nystagmus recovery after injury. These data indicates the possibility that intracochlear administration of steroid and ATP to animals with acute vestibular lesion may lead to rapid correction of vestibular imbalance.2 Establishment of vestibular function analysisA new eye movement image analysis technique was devised. With this technique, we can calculate maximum slow phase velocity. We have shown the relation between post-rotatory nystagmus number and maximum slow phase velocity. Furthermore, using this technique, we can calculate vestibulo-ocular reflex gain. Various kinds of drug effects on vestibular function can be investigated with no invasion.

1药物对前庭功能障碍的影响观察类固醇和三磷酸腺苷对前庭功能障碍豚鼠外周前庭系统的影响。手术横断右侧半规管，抽吸淋巴液2秒钟。切断端用纤维蛋白胶封闭。耳蜗术后，用渗透泵将倍他米松或三磷酸腺苷直接注入鼓阶。治疗前后分别进行梯形旋转试验，并在黑暗中用CCD摄像机将旋转后眼球震颤记录在录像带上，进行分析。倍他米松和三磷酸腺苷均能显著促进损伤后眼球震颤的恢复。这些数据表明，对于急性前庭损害的动物，在前庭皮质内给予类固醇和三磷酸腺苷可能导致前庭失衡的快速纠正。2前庭功能分析的建立一种新的眼动图像分析技术。利用这一技术，我们可以计算出最大慢相速度。我们已经证明了旋转后眼震数目与最大慢相速度的关系。此外，使用这项技术，我们可以计算前庭-眼睛反射增益。各种药物对前庭功能的影响可以在无创的情况下进行研究。