Recovery of vestibular function by intracochlear administration of various kinds of agents

研究了类固醇和ATP对前庭功能障碍豚鼠外周前庭系统的影响。手术切除右侧半规管，并抽吸淋巴液2秒。横切的两端用纤维蛋白胶密封。耳蜗切开术后，用渗透泵直接输注倍他米松或ATP。治疗前后行梯形旋转试验，在黑暗中用CCD摄像机记录旋转后眼球震颤，并进行分析。倍他米松和ATP均能显著改善损伤后旋转性眼球震颤的恢复。这些数据表明，急性前庭病变动物在耳蜗内给予类固醇和ATP可能导致前庭失衡的快速纠正前庭功能分析的建立设计了一种新的眼动图像分析技术。利用这种方法，我们可以计算出最大慢相速度。我们展示了旋转后眼震数与最大慢相速度之间的关系。此外，利用该技术，我们可以计算前庭-眼反射增益。各种药物对前庭功能的影响可以在不侵犯的情况下进行研究。

1 Drug effects on vestibular disorderEffects of steroid and ATP on peripheral vestibular system in guinea pig with vestibular disorder were investigated. The right lateral semicircular canal was surgically transected and lymph fluids were suctioned for two seconds. The transected ends were sealed with fibrin glue. After cochleotomy, betamethasone or ATP was directly infused into scala tympany with an osmotic pump. Before and after treatment, trapezoid rotation tests were performed and post-rotatory nystagmus was recorded on videotape with CCD camera in the dark, and was analyzed. Both betamethasone and ATP significantly improved post-rotatory nystagmus recovery after injury. These data indicates the possibility that intracochlear administration of steroid and ATP to animals with acute vestibular lesion may lead to rapid correction of vestibular imbalance.2 Establishment of vestibular function analysisA new eye movement image analysis technique was devised. With this technique, we can calculate maximum slow phase velocity. We have shown the relation between post-rotatory nystagmus number and maximum slow phase velocity. Furthermore, using this technique, we can calculate vestibulo-ocular reflex gain. Various kinds of drug effects on vestibular function can be investigated with no invasion.