Direct administration therapy for irreversible peripheral vestibular disorder

我们评估了自由基清除剂依达拉奉对链霉素（SM）或AMPA 1诱导的前庭障碍的影响。用渗透泵将30%SM注入豚鼠右内耳24 h，造成豚鼠右内耳损伤。依达拉奉全身给药可改善豚鼠在损伤后3天和7天的前庭眼反射增益。局部应用依达拉奉可抑制豚鼠损伤后观察到的自发性眼球震颤频率2。通过使用注射器泵输注AMPA（10 mM，50 μl）5 min，在豚鼠右内耳中造成损伤。AMPA输注后，观察到自发性眼震，同时输注CNQX，一种有效的AMPA受体拮抗剂，抑制眼震的频率，以剂量依赖性的方式。局部应用依达拉奉也能抑制眼震，而且早期（12 h内）应用依达拉奉可改善损伤后1周对热刺激的反应，因此，局部应用依达拉奉可能是治疗氨基糖苷类或谷氨酸兴奋性毒性所致前庭功能障碍的一种方法

We evaluated the effects of edaravone, a free radical scavenger, on the vestibular disorder induced by streptomycin (SM) or AMPA1. Lesions were made in the guinea pig right inner ear by intracochlear administration of 30% SM for 24 h using osmotic pump. Systemic administration of edaravone improved vestibulo-ocular reflex gain in guinea pigs at 3 and 7 days after lesions were made. Local application of edaravone suppressed the frequency of spontaneous nystagmus observed in guinea pigs after lesions were made2. Lesions were made in the guinea pig right inner ear by infusion of AMPA (10 mM, 50 μl) for 5 min using syringe pump. After AMPA infusion, spontaneous nystagmus was observed, and simultaneous infusion of CNQX, a potent AMPA receptor antagonist, suppressed the frequency of nystagmus in a dose-dependent manner. Local application of edaravone also suppressed the nystagmus, furthermore, early (within 12 h) application of edaravone improved a response for caloric stimulation at 1 w after lesions were madeWe concluded that local application of edaravone may be one of the treatment for vestibular disorder caused by aminoglycoside or glutamate excitotoxicity